Pseudomonas aeruginosa reverse diauxie is a multidimensional, optimized, resource utilization strategy.
Pseudomonas aeruginosa reverse diauxie is a multidimensional, optimized, resource utilization strategy.
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铜绿假单胞菌逆向二次代谢是一种多维的、优化的资源利用策略。
DOI：
10.1038/s41598-020-80522-8
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发表时间：
2021-01-14
影响因子：
4.6
通讯作者：
Carlson RP
中科院分区：
文献类型：
--
作者：
McGill SL;Yung Y;Hunt KA;Henson MA;Hanley L;Carlson RP
铜绿假单胞菌是一种全球分布的细菌，常见于医学感染中。这种条件致病菌使用一种不同于许多模型微生物的碳分解代谢抑制(CCR)策略。它不利用经典的二氧体表型，也不遵循常见的系统生物学假设，包括优先消耗葡萄糖和‘溢出’新陈代谢。尽管存在这些矛盾，但铜绿假单胞菌在许多不同的环境中都具有竞争力，这突显了微生物生态学和系统生物学方面的知识差距。生理学、组学和In Silico分析被用来量化铜绿假单胞菌的CCR策略，即“反向二倍体”。利用基因组规模的代谢模型，结合体外组学数据，确定了反向二倍体的生态学基础。使用一种多维策略预测了对较低能量、不可发酵的碳源(如醋酸盐或琥珀酸而不是葡萄糖)的反向二重偏爱，该策略在完全氧化底物的同时最大限度地减少了对中心代谢的资源投资。在计算机优化标准中应用一种常见的最大化生长速度的标准，并不能预测反向二氧杂表型。这项研究量化了铜绿假单胞菌广泛分布和毒力的基础代谢策略，包括它与环境和医学感染中常见的微生物之间潜在的、相互作用的相互作用。
Pseudomonas aeruginosa is a globally-distributed bacterium often found in medical infections. The opportunistic pathogen uses a different, carbon catabolite repression (CCR) strategy than many, model microorganisms. It does not utilize a classic diauxie phenotype, nor does it follow common systems biology assumptions including preferential consumption of glucose with an ‘overflow’ metabolism. Despite these contradictions, P. aeruginosa is competitive in many, disparate environments underscoring knowledge gaps in microbial ecology and systems biology. Physiological, omics, and in silico analyses were used to quantify the P. aeruginosa CCR strategy known as ‘reverse diauxie’. An ecological basis of reverse diauxie was identified using a genome-scale, metabolic model interrogated with in vitro omics data. Reverse diauxie preference for lower energy, nonfermentable carbon sources, such as acetate or succinate over glucose, was predicted using a multidimensional strategy which minimized resource investment into central metabolism while completely oxidizing substrates. Application of a common, in silico optimization criterion, which maximizes growth rate, did not predict the reverse diauxie phenotypes. This study quantifies P. aeruginosa metabolic strategies foundational to its wide distribution and virulence including its potentially, mutualistic interactions with microorganisms found commonly in the environment and in medical infections.
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影响因子：
3.7
作者：
Dalton T;Dowd SE;Wolcott RD;Sun Y;Watters C;Griswold JA;Rumbaugh KP
通讯作者：
Rumbaugh KP
影响因子：
3.7
作者：
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通讯作者：
Neves AR
影响因子：
3.2
作者：
Folsom, James Patrick;Parker, Albert E.;Carlson, Ross P.
通讯作者：
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影响因子：
64.8
作者：
Basan M;Hui S;Okano H;Zhang Z;Shen Y;Williamson JR;Hwa T
通讯作者：
Hwa T
影响因子：
3.2
作者：
Filkins, Laura M.;Graber, Jyoti A.;O'Toole, George A.
通讯作者：
O'Toole, George A.