Effect of oxygen on glucose metabolism: utilization of lactate in Staphylococcus aureus as revealed by in vivo NMR studies.
Effect of oxygen on glucose metabolism: utilization of lactate in Staphylococcus aureus as revealed by in vivo NMR studies.
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DOI：
10.1371/journal.pone.0058277
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Neves AR
中科院分区：
文献类型：
--
作者：
Ferreira MT;Manso AS;Gaspar P;Pinho MG;Neves AR
成功适应不断变化的寄主条件的能力对于细菌病原体的完全毒力至关重要。金黄色葡萄球菌在感染过程中必须应对氧气浓度的波动。因此，我们利用活体~(13)C-核磁共振技术研究了氧对非生长金黄色葡萄球菌COL-S细胞葡萄糖代谢的影响。在有氧(直接影响新陈代谢)或厌氧(对缺氧的转录调节)生长的细胞悬浮液中，检测了不同氧浓度下的葡萄糖分解代谢。在有氧培养的细胞中，葡萄糖消耗的速率随着氧气浓度的降低而递减。此外，缺氧导致双相葡萄糖消耗，第二阶段表现出更高的比率。1，6-二磷酸果糖池在葡萄糖仍然丰富时达到峰值，但瞬时最大值随氧气浓度的变化而变化。当氧气变得受限时，检测到甘露醇/甘露醇-1-磷酸作为葡萄糖分解代谢的产物。在缺氧条件下，甘露醇-1-磷酸的积累随着转换到更高的葡萄糖消耗率而停止，这意味着激活了一种更有效的方式来再生NAD+。葡萄糖分解代谢的最终产物分布受氧气的影响很大：乙酸盐随氧浓度的增加而增加，乳酸随氧浓度的增加而减少；乙醇仅在厌氧条件下形成。此外，氧气促进了乳酸转化为醋酸盐的有利能量，这在全氧条件下尤其明显。有趣的是，在有氧生长的情况下，金黄色葡萄球菌细胞也将乳酸转化为醋酸盐，同时使用葡萄糖和乳酸作为生长的底物，并在乳酸培养基上生长得相当好。我们认为，高效的乳酸分解代谢可能使金黄色葡萄球菌在其生态位上具有代谢优势。
The ability to successfully adapt to changing host conditions is crucial for full virulence of bacterial pathogens. Staphylococcus aureus has to cope with fluctuating oxygen concentrations during the course of infection. Hence, we studied the effect of oxygen on glucose metabolism in non-growing S. aureus COL-S cells by in vivo 13C-NMR. Glucose catabolism was probed at different oxygen concentrations in suspensions of cells grown aerobically (direct effects on metabolism) or anaerobically (transcriptional adjustment to oxygen deprivation). In aerobically-grown cells, the rate of glucose consumption diminished progressively with decreasing oxygen concentrations. Additionally, oxygen deprivation resulted in biphasic glucose consumption, with the second phase presenting a higher rate. The fructose-1,6-bisphosphate pool peaked while glucose was still abundant, but the transient maximum varied with the oxygen concentration. As oxygen became limiting mannitol/mannitol-1-phosphate were detected as products of glucose catabolism. Under anoxic conditions, accumulation of mannitol-1-phosphate ceased with the switch to higher glucose consumption rates, which implies the activation of a more efficient means by which NAD+ can be regenerated. The distribution of end-products deriving from glucose catabolism was dramatically affected by oxygen: acetate increased and lactate decreased with the oxygen concentration; ethanol was formed only anaerobically. Moreover, oxygen promoted the energetically favourable conversion of lactate into acetate, which was particularly noticeable under fully oxygenated conditions. Interestingly, under aerobiosis growing S. aureus cells also converted lactate to acetate, used simultaneously glucose and lactate as substrates for growth, and grew considerably well on lactate-medium. We propose that the efficient lactate catabolism may endow S. aureus with a metabolic advantage in its ecological niche.
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影响因子：
6.7
作者：
Friedman DB;Stauff DL;Pishchany G;Whitwell CW;Torres VJ;Skaar EP
通讯作者：
Skaar EP
影响因子：
3.2
作者：
Ferain, T;Schanck, AN;Delcour, J
通讯作者：
Delcour, J
DOI：
10.1007/s004380051001
发表时间：
1999-04-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Gertz, S;Engelmann, S;Hecker, M
通讯作者：
Hecker, M
影响因子：
--
作者：
Liebeke, Manuel;Doerries, Kirsten;Lalk, Michael
通讯作者：
Lalk, Michael
DOI：
10.1099/00221287-29-1-157
发表时间：
1962-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
GARDNER, JF;LASCELLES, J
通讯作者：
LASCELLES, J