Developing an In Vitro Model to Screen Drugs for Nerve Regeneration.
Developing an In Vitro Model to Screen Drugs for Nerve Regeneration.
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DOI：
10.1002/ar.23918
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Phillips JB
中科院分区：
文献类型：
--
作者：
Rayner MLD;Laranjeira S;Evans RE;Shipley RJ;Healy J;Phillips JB
周围神经损伤（PNI）发病率高，可使人衰弱，导致终末器官功能丧失或紊乱，从而影响患者的生活质量。目前的治疗方法使用显微外科手术方法，但有可能通过其他治疗方式增强恢复，例如；基于细胞的导管，基因治疗和小分子。许多具有改善神经再生潜力的分子靶点和药物已经被确定，然而，在将治疗方法转向临床转化方面存在挑战。由于缺乏对潜在药物治疗的促再生作用的详细了解，因此需要有效的体外模型来筛选化合物，为未来的临床前和临床研究提供信息。再生神经突和支持雪旺细胞之间的相互作用是神经环境的一个关键特征，因此，模拟这种细胞关联的体外模型是有用的工具。在这项研究中，我们研究了各种细胞培养模型，包括简单的单层系统和更复杂的3D工程共培养，作为用于PNI药物开发的模型。中国生物医学工程学报，2018,31(1):1628 - 1637。©2018作者。由Wiley期刊公司代表美国解剖学家协会出版的解剖学记录。
Peripheral nerve injuries (PNI) have a high prevalence and can be debilitating, resulting in life‐long loss or disturbance in end‐organ function, which compromises quality of life for patients. Current therapies use microsurgical approaches but there is the potential for enhancing recovery through other therapeutic modalities such as; cell‐based conduits, gene therapy and small molecules. A number of molecular targets and drugs which have the potential to improve nerve regeneration have been identified, however, there are challenges associated with moving therapies toward clinical translation. Due to the lack of detailed knowledge about the pro‐regenerative effect of potential drug treatments, there is a need for effective in vitro models to screen compounds to inform future pre‐clinical and clinical studies. The interaction between regenerating neurites and supporting Schwann cells is a key feature of the nerve environment, therefore, in vitro models that mimic this cellular association are useful tools. In this study, we have investigated various cell culture models, including simple monolayer systems and more complex 3D‐engineered co‐cultures, as models for use in PNI drug development. Anat Rec, 301:1628–1637, 2018. © 2018 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.
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影响因子：
4.1
作者：
East, Emma;de Oliveira, Daniela Blum;Phillips, James B.
通讯作者：
Phillips, James B.
影响因子：
4.2
作者：
Edoff, K;Jerregård, H
通讯作者：
Jerregård, H
影响因子：
2.8
作者：
Clarke, Joseph C.;Tuft, Bradley W.;Clinger, John D.;Levine, Rachel;Figueroa, Lucas Sievens;Guymon, C. Allan;Hansen, Marlan R.
通讯作者：
Hansen, Marlan R.
影响因子：
4.9
作者：
Ahmed, I;Liu, HY;Meiners, S
通讯作者：
Meiners, S
DOI：
10.1523/jneurosci.5045-09.2010
发表时间：
2010-01-20
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dill J;Patel AR;Yang XL;Bachoo R;Powell CM;Li S
通讯作者：
Li S