Different timings of Dicer deletion affect neurogenesis and gliogenesis in the developing mouse central nervous system.
Different timings of Dicer deletion affect neurogenesis and gliogenesis in the developing mouse central nervous system.
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DOI：
10.1002/dvdy.22109
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发表时间：
2009-11
影响因子：
2.5
通讯作者：
Sun, Tao
中科院分区：
文献类型：
--
作者：
Kawase-Koga, Yoko;Otaegi, Gaizka;Sun, Tao
MicroRNAs是由RNAase III酶Dester加工的约22个核苷酸的内源性非编码小RNA。目前，Dester在小鼠中枢神经系统(CNS)发育过程中的作用尚不清楚。在这里，我们表明，使用两个Cre系特异性地删除中枢神经系统和大脑皮层中DICER的表达会导致祖细胞数量减少、神经元分化异常和皮质壁变薄。胚胎早期不完全缺失有助于早期出生的皮质神经元和脊髓运动神经元的正常发育。然而，在胚胎晚期，当中枢神经系统完全消融时，皮质中晚出生神经元的迁移和脊髓中少突胶质细胞前体的扩张和分化受到很大影响。我们对不同时间的DICER缺失的研究证明了DICER介导的microRNA通路在调节中枢神经系统发育过程中神经发生和神经胶质发生的不同阶段中的重要性。
MicroRNAs, processed by the RNAase III enzyme Dicer, are ~22 nucleotide endogenous noncoding small RNAs. The function of Dicer in the mouse central nervous system (CNS) development is not well understood. Here we show that specifically deleting Dicer expression in the CNS and in the cerebral cortex using two Cre lines results in reduced progenitor numbers, abnormal neuronal differentiation and thinner cortical wall. Incomplete Dicer deletion during early embryonic stages contributes to normal development of early-born neurons in the cortex and motor neurons in the spinal cord. However, at late embryonic stages when Dicer is completely ablated in the CNS, the migration of late-born neurons in the cortex and oligodendrocyte precursor expansion and differentiation in the spinal cord are greatly affected. Our studies of different timings of Dicer deletion demonstrate the importance of the Dicer-mediated microRNA pathway in regulating distinct phases of neurogenesis and gliogenesis during the CNS development.
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影响因子：
5.3
作者：
Davis, Tigwa H.;Cuellar, Trinna L.;Ullian, Erik M.
通讯作者：
Ullian, Erik M.
DOI：
10.1073/pnas.0801689105
发表时间：
2008-04-08
影响因子：
11.1
作者：
Cuellar, Trinna L.;Davis, Tigwa H.;McManus, Michael T.
通讯作者：
McManus, Michael T.
影响因子：
64.5
作者：
LEE, RC;FEINBAUM, RL;AMBROS, V
通讯作者：
AMBROS, V
影响因子：
30.8
作者：
Bernstein, E;Kim, SY;Hannon, GJ
通讯作者：
Hannon, GJ
DOI：
10.1073/pnas.0504834102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Harfe, BD;McManus, MT;Tabin, CJ
通讯作者：
Tabin, CJ