Antibodies Against Synthetic Peptides-猫眼课题宝

合成肽抗体

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发表时间：

1983

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作者：

G. Walter;R. Doolittle

20 世纪 60 年代初引入的简单高效的肽合成方法 (1) 为生物学家和蛋白质化学家提供了强大的研究工具。因此，不仅可以合成生物活性肽，而且可以合成蛋白质的离散片段。从一开始就很明显，这项技术可以与长期存在的免疫程序相结合，从而将小分子用作抗原材料 (2)。事实上，该方法很快就被应用于许多蛋白质，以寻找哪些特征使它们具有抗原性 (3)。不过，可以公平地说，免疫学和合成技术相结合的技术在 20 世纪 60 年代和 1970 年代从未得到充分利用。从那时起，DNA 测序领域的进步提供了大量有关蛋白质的序列信息，无论是真实的还是推定的，其中许多从未被分离出来，更不用说表征了。序列数据可以以多种方式利用，特别是与计算机辅助预测方案结合使用，该方案允许对给定序列的三维结构进行一定程度的有意义的推测。 DNA/蛋白质序列数据可用于的目的之一是化学合成与蛋白质部分相对应的肽。

The introduction of a simple and efficient peptide synthesis methodology in the early 1960’s (1) put a powerful research tool at the disposal of biologists and protein chemists alike. Thus, it was immediately feasible to synthesize not only biologically active peptides, but also discrete segments of proteins. It was obvious from the start, also, that this technology could be coupled with longstanding immunologic procedures whereby small molecules can be used as antigenic materials (2). Indeed, the approach was soon applied to a number of proteins in a quest to find what features made them antigenic (3). It is fair to say, though, that the full capacity of the combined immunologic and synthetic techniques was never exploited during the 1960’s and 1970’s. Since then advances in the field of DNA sequencing have provided a plethora of sequence information about proteins, real and putative, many of which have never been isolated, never mind characterized. The sequence data can be utilized in a variety of ways, especially in conjunction with computer-aided predictive schemes that allow a degree of meaningful conjecture about the three-dimensional structure of a given sequence. Among the ends to which DNA/protein sequence data can be put is the chemical synthesis of peptides corresponding to portions of the protein.