RNAi-based screens uncover a potential new role for the orphan neuropeptide receptor Moody in Drosophila female germline stem cell maintenance.
RNAi-based screens uncover a potential new role for the orphan neuropeptide receptor Moody in Drosophila female germline stem cell maintenance.
复制标题
DOI：
10.1371/journal.pone.0243756
复制
发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Drummond-Barbosa D
中科院分区：
文献类型：
--
作者：
Ma T;Matsuoka S;Drummond-Barbosa D
生殖对生理和外部环境的变化高度敏感。神经肽是进化上保守的信号分子，调节多种生理过程。然而，许多神经肽信号通路的潜在生殖作用仍未得到充分研究。在这里，我们描述了基于RNAi的黑腹果蝇筛选结果，以确定在卵子发生中具有潜在作用的神经肽/神经肽受体。屏幕读数要么是每只雌性在一段时间内每天产卵的数量，要么是解剖卵巢的荧光显微镜分析。我们发现，由Moody(与哺乳动物褪黑激素受体同源)编码的孤儿神经肽受体可能是体细胞正常产卵和适当的生殖系干细胞维持所必需的。然而，产卵屏幕的信噪比较低，并未导致识别出更多的候选对象。因此，尽管卵子计数分析可能有助于大规模筛选识别导致卵子发生戏剧性变化的卵子发生调控因子，但更劳动密集型的基于显微镜的筛查更适合于识别具有更微妙表型的新的卵子发生生理调控因子。
Reproduction is highly sensitive to changes in physiology and the external environment. Neuropeptides are evolutionarily conserved signaling molecules that regulate multiple physiological processes. However, the potential reproductive roles of many neuropeptide signaling pathways remain underexplored. Here, we describe the results of RNAi-based screens in Drosophila melanogaster to identify neuropeptides/neuropeptide receptors with potential roles in oogenesis. The screen read-outs were either the number of eggs laid per female per day over time or fluorescence microscopy analysis of dissected ovaries. We found that the orphan neuropeptide receptor encoded by moody (homologous to mammalian melatonin receptors) is likely required in somatic cells for normal egg production and proper germline stem cell maintenance. However, the egg laying screens had low signal-to-noise ratio and did not lead to the identification of additional candidates. Thus, although egg count assays might be useful for large-scale screens to identify oogenesis regulators that result in dramatic changes in oogenesis, more labor-intensive microscopy-based screen are better applicable for identifying new physiological regulators of oogenesis with more subtle phenotypes.
登录
查看更多内容
影响因子：
4.5
作者：
Ameku T;Niwa R
通讯作者：
Niwa R
DOI：
10.1016/j.beem.2017.11.001
发表时间：
2017-12-01
影响因子：
7.4
作者：
Gizowski, Claire;Trudel, Eric;Bourque, Charles W.
通讯作者：
Bourque, Charles W.
影响因子：
64.5
作者：
Bainton, RJ;Tsai, LTY;Heberlein, U
通讯作者：
Heberlein, U
影响因子：
9.8
作者：
Ameku T;Yoshinari Y;Texada MJ;Kondo S;Amezawa K;Yoshizaki G;Shimada-Niwa Y;Niwa R
通讯作者：
Niwa R
DOI：
10.1016/j.pnpbp.2015.02.006
发表时间：
2016-02-04
影响因子：
5.6
作者：
Barson JR;Leibowitz SF
通讯作者：
Leibowitz SF