Midgut-derived neuropeptide F controls germline stem cell proliferation in a mating-dependent manner.
Midgut-derived neuropeptide F controls germline stem cell proliferation in a mating-dependent manner.
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DOI：
10.1371/journal.pbio.2005004
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发表时间：
2018-09
期刊：
影响因子：
9.8
通讯作者：
Niwa R
中科院分区：
文献类型：
--
作者：
Ameku T;Yoshinari Y;Texada MJ;Kondo S;Amezawa K;Yoshizaki G;Shimada-Niwa Y;Niwa R
干细胞维持是由促进干细胞自我更新的邻近生态位细胞建立的。然而，人们对干细胞活性如何通过系统、组织外在信号响应环境线索和生理状态变化进行调节还知之甚少。在这里，我们发现神经肽 F (NPF) 信号在调节果蝇交配诱导的生殖干细胞 (GSC) 增殖的途径中发挥着重要作用。中肠肠内分泌细胞（EEC）中表达的 NPF 在交配时响应精液蛋白性肽（SP）而释放。这种源自中肠的 NPF 通过卵巢 NPF 受体 (NPFR) 活性控制交配诱导的 GSC 增殖，从而调节 GSC 中的骨形态发生蛋白 (BMP) 信号水平。我们的研究提供了一种分子机制，描述了肠道衍生的系统因子如何通过器官间通讯将干细胞行为与生理状态（例如交配）结合起来。不同器官之间的通信对于快速响应环境线索或生物体生理状态的变化至关重要。最近的研究表明体液因子或激素的存在，它们通过循环系统输送到不同的器官并实现它们之间的协调。在这里，我们分析了果蝇果蝇卵巢中调节生殖干细胞（GSC）增殖的器官之间的通讯机制。我们证明，一种名为神经肽 F (NPF) 的肽激素在这一过程中发挥着关键作用。这种肽在大脑和中肠中产生，值得注意的是，我们发现只有中肠释放的 NPF 对于控制交配后 GSC 增殖至关重要。我们的数据表明，交配会刺激中肠内分泌细胞释放 NPF，而中肠内分泌细胞受精肽的存在刺激。然后，中肠来源的 NPF 通过卵巢中表达的 NPF 特异性 G 蛋白偶联受体进行转导，从而触发 GSC 增殖。我们的研究确定了消化系统和卵巢之间的重要相互作用，该相互作用根据交配调节果蝇干细胞群的大小。
Stem cell maintenance is established by neighboring niche cells that promote stem cell self-renewal. However, it is poorly understood how stem cell activity is regulated by systemic, tissue-extrinsic signals in response to environmental cues and changes in physiological status. Here, we show that neuropeptide F (NPF) signaling plays an important role in the pathway regulating mating-induced germline stem cell (GSC) proliferation in the fruit fly Drosophila melanogaster. NPF expressed in enteroendocrine cells (EECs) of the midgut is released in response to the seminal-fluid protein sex peptide (SP) upon mating. This midgut-derived NPF controls mating-induced GSC proliferation via ovarian NPF receptor (NPFR) activity, which modulates bone morphogenetic protein (BMP) signaling levels in GSCs. Our study provides a molecular mechanism that describes how a gut-derived systemic factor couples stem cell behavior to physiological status, such as mating, through interorgan communication. Communication between different organs is essential to respond quickly to environmental cues or changes in the physiological status of an organism. Recent studies have shown the existence of humoral factors or hormones, which are transported by the circulatory system to different organs and achieve coordination between them. Here, we have analyzed the communication mechanism between organs that regulates proliferation of germline stem cells (GSCs) in the ovary of the fruit fly Drosophila melanogaster. We show that a peptide hormone called neuropeptide F (NPF) is a key player in this process. This peptide is produced in both the brain and the midgut, and, remarkably, we find that only NPF released from the midgut is crucial for controlling post-mating GSC proliferation. Our data suggest that mating stimulates the release of NPF from the endocrine cells of the midgut stimulated by the presence of a seminal peptide. Midgut-derived NPF is then transduced through an NPF-specific G-protein–coupled receptor expressed in the ovary, and this triggers GSC proliferation. Our study identifies an essential interaction between the digestive system and the ovary that regulates the size of stem cell populations in flies depending on mating.
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影响因子：
29
作者：
Beshel J;Dubnau J;Zhong Y
通讯作者：
Zhong Y
DOI：
10.1073/pnas.90.6.2512
发表时间：
1993-03-15
影响因子：
11.1
作者：
DING, D;PARKHURST, SM;LIPSHITZ, HD
通讯作者：
LIPSHITZ, HD
影响因子：
4.5
作者：
Ameku T;Niwa R
通讯作者：
Niwa R
影响因子：
23.9
作者：
Ables, Elizabeth T.;Drummond-Barbosa, Daniela
通讯作者：
Drummond-Barbosa, Daniela
DOI：
10.1073/pnas.0809144106
发表时间：
2009-01-27
影响因子：
11.1
作者：
Hsu, Hwei-Jan;Drummond-Barbosa, Daniela
通讯作者：
Drummond-Barbosa, Daniela