Foetal and cancer patient fibroblasts produce an autocrine migration-stimulating factor not made by normal adult cells.
Foetal and cancer patient fibroblasts produce an autocrine migration-stimulating factor not made by normal adult cells.
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胎儿和癌症患者的成纤维细胞产生正常成人细胞不产生的自分泌迁移刺激因子。
作者：
S. Schor;A. Schor;A. Grey;G. Rushton
我们以前曾报道过（1）胎儿和成人成纤维细胞迁移到三维胶原基质中受到细胞密度的不同影响，（2）癌症患者的皮肤成纤维细胞通常显示出胎儿样的迁移行为模式。本文提供的数据表明，这些细胞类型的迁移差异在以高密度（即细胞汇合）铺板的培养物中特别明显;在这些条件下，胎儿成纤维细胞和癌症患者的胎儿样成纤维细胞迁移到三维胶原基质中的程度显著大于正常成人细胞。在这一初步研究有关的生化基础上，这些意见，我们报告说，无论是胎儿或癌症患者成纤维细胞条件培养基刺激融合的成年细胞的迁移。这种迁移刺激对融合细胞是特异性的，因为亚融合成体成纤维细胞的迁移不受这些条件培养基的影响。胎儿成纤维细胞条件培养基的凝胶过滤色谱表明，迁移刺激活性以单峰形式恢复，表观分子量在50-60（× 10（3））范围内。胎儿和癌症患者成纤维细胞条件培养基中的活性迁移刺激因子（MSF）似乎是在酸性pH下稳定的蛋白质，但通过热、碱性pH和还原性烷基化而失活。由胎儿和癌症患者成纤维细胞产生的MSF可能是这些细胞在融合培养物中显示的迁移水平升高的特征性原因，从而表明该因子的自分泌作用模式。（250字处删节）
We have previously reported that (1) the migration of foetal and adult fibroblasts into three-dimensional collagen matrices is differentially affected by cell density, and (2) skin fibroblasts from cancer patients commonly display a foetal-like mode of migratory behaviour. Data presented here indicate that differences in the migration of these cell types are particularly apparent in cultures plated at high density (i.e. at cell confluence); under these conditions, foetal fibroblasts and the foetal-like fibroblasts of cancer patients migrate into the three-dimensional collagen matrix to a significantly greater extent than do normal adult cells. In this initial study concerned with the biochemical basis of these observations, we report that medium conditioned by either foetal or cancer patient fibroblasts stimulates the migration of confluent adult cells. This stimulation of migration is specific to confluent cells, as the migration of subconfluent adult fibroblasts is unaffected by these conditioned media. Gel filtration chromatography of foetal fibroblast-conditioned medium indicates that migration-stimulating activity is recovered in a single peak with an apparent molecular mass in the range of 50-60 (X 10(3]. The active migration stimulating factor (MSF) in both foetal and cancer patient fibroblast-conditioned media appears to be a protein stable at acid pH, but inactivated by heat, alkaline pH and reductive alkylation. MSF produced by foetal and cancer patient fibroblasts is presumably responsible for the characteristically elevated levels of migration displayed by these cells in confluent culture, thereby suggesting an autocrine mode of action for this factor.(ABSTRACT TRUNCATED AT 250 WORDS)
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DOI：
--
发表时间：
1986-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;J. Massagué
通讯作者：
R. Ignotz;J. Massagué
大鼠乳腺癌细胞克隆对肿瘤源性细胞因子的趋化反应。
DOI：
10.1016/0006-291x(87)90746-7
发表时间：
1987
影响因子：
3.1
作者：
Atnip,KD;Carter,LM;Nicolson,GL;Dabbous,MK
通讯作者：
Dabbous,MK
影响因子：
10.3
作者：
Johnco, C.;Wuthrich, V. M.;Rapee, R. M.
通讯作者：
Rapee, R. M.
培养的血管细胞响应血浆和血小板衍生因子的迁移。
影响因子：
4
作者：
Bernstein,LR;Antoniades,H;Zetter,BR
通讯作者：
Zetter,BR