Oxidative stress protective function of ApY2SK2 dehydrin: a late embryogenesis abundant protein in embryogenic callus of Agapanthus praecox to promote post-cryopreservation survival
Oxidative stress protective function of ApY2SK2 dehydrin: a late embryogenesis abundant protein in embryogenic callus of Agapanthus praecox to promote post-cryopreservation survival
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ApY2SK2脱水蛋白的氧化应激保护功能：百子莲胚胎发生愈伤组织中胚胎发生后期丰富的蛋白质，可促进冷冻保存后的存活
DOI：
10.1007/s11240-022-02261-0
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Di Zhang
中科院分区：
文献类型：
--
作者：
Tao Liu;Yiying Zhang;Yunxia Chu;Hairong Chen;Li Ren;Di Zhang
脱氢蛋白(DHNS)作为胚胎发育后期丰富蛋白家族的成员，在种子脱水保护和植物对多种非生物胁迫的适应中发挥着重要作用。超低温保存作为种质保存的一种重要方法，也是研究复合逆境的理想研究体系。氧化应激作为低温保存过程中的关键应激，直接影响细胞的活力。我们之前的体外实验表明，ApY2SK2 dhn能有效地保护酶活性，使低温保存后的拟南芥幼苗的存活率提高近一倍，但ApY2SK2对低温保存的体内保护作用尚未阐明。本研究通过过表达(OE)和RNA干扰(RNAi)技术，将ApY2SK2型DHN基因导入仙人掌胚性愈伤组织(EC)，研究DHN在超低温保存过程中对体内氧化应激的保护作用。结果表明，OE细胞和RNAi细胞的细胞活力变化趋势完全相反，冻存后ApY2SK2-OE EC细胞相对死亡率显著降低18.5%，ApY2SK2-RNAi细胞相对死亡率显著提高23.5%。在超低温保存过程中，过表达ApY2SK2提高了非酶类抗氧化剂(AsA和GSH)含量、抗氧化酶(POD和SOD)活性，上调了CAT、POD和GPx的表达，而ApY2SK2-RNAi细胞则降低了CAT、FeSOD、POD和GPx的表达。这些结果表明，ApY2SK2可以影响植物细胞在超低温保存过程中的ROS代谢，减轻H_2O_2和OH·的过量产生，激活抗氧化系统，改善细胞氧化还原平衡，减轻膜脂过氧化损伤。脱氢表雄酮能有效地提高细胞的抗逆性，在植物体内或体外超低温保存方面具有很大的应用潜力。ApY2SK2脱水蛋白在低温保存过程中可以影响ROS代谢，激活抗氧化系统，减少膜脂过氧化，从而提高复苏后的存活率。
Dehydrins (DHNs) as the member of the late embryogenesis abundant protein family, play critical roles in seed dehydration protection and plant adaptation to multiple abiotic stresses. As an important method of germplasm preservation, cryopreservation is also an ideal research system to study compound stress. Oxidative stress, as the critical stress in cryopreservation, directly affects cell viability. Our previous in vitro tests indicated that ApY2SK2 DHN can effectively protect enzyme activity and almost double the survival rate of Arabidopsis thaliana seedlings after cryopreservation, but the in vivo protective effect of ApY2SK2 on cryopreservation have not yet been elucidated. In this study, ApY2SK2 type DHN was genetically transformed into embryogenic callus (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo oxidative stress protective effect of DHNs during cryopreservation. The results showed that the cell viability had a completely opposite trend between OE and RNAi cell lines, and the cell relative death ratio of ApY2SK2-OE EC was significantly decreased 18.5% and ApY2SK2-RNAi cells was significantly increased 23.5% after cryopreservation. Overexpression ApY2SK2 increased non-enzymatic antioxidant (AsA and GSH) contents, antioxidant enzyme (POD and SOD) activities and up-regulated CAT, POD and GPX expression, while ApY2SK2-RNAi cells decreased CAT, FeSOD, POD and GPX expression during cryopreservation. These findings suggested that ApY2SK2 can affect ROS metabolism, alleviate H2O2 and OH·excessive generation, activate the antioxidant system, improve cellular REDOX balance and reduce membrane lipid peroxidation damage of plant cells during cryopreservation. DHNs can effectively improve cell stress tolerance and have great potential for in vivo or in vitro applications in plant cryopreservation. ApY2SK2 dehydrin can affect ROS metabolism, activate the antioxidant system and reduce membrane lipid peroxidation during cryopreservatin leading to high survival rate after recovery.
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影响因子：
5.1
作者：
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影响因子：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
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发表时间：
2013-11
期刊：
Plant science : an international journal of experimental plant biology
影响因子：
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