Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus.
Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus.
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单壁碳纳米管提高百子莲预胚愈伤组织细胞存活率并减少氧化损伤
DOI：
10.1186/s13007-020-00674-6
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发表时间：
2020
期刊：
影响因子：
5.1
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Ren L;Deng S;Chu Y;Zhang Y;Zhao H;Chen H;Zhang D
超低温保存是植物种质资源离体长期保存的最佳方法。初步研究发现，活性氧（ROS）诱导的氧化应激和冰诱导的膜损伤是冻存样品中细胞死亡的根本原因。如何提高植物超低温保存的成活率是低温生物学领域的一个重要科学问题。在植物玻璃化液（PVS）中添加单壁碳纳米管（SWCNTs）可显著提高百子莲胚性愈伤组织（EC）超低温保存的存活率，并分析了对照和添加SWCNTs超低温保存过程中细胞的氧化反应。单壁碳纳米管在脱水阶段进入EC，主要分布在细胞壁周围和囊泡中，在稀释阶段大部分单壁碳纳米管移出EC。结合生理指标和基因定量表达结果，单壁碳纳米管影响植物超低温保存过程中ROS信号转导和抗氧化系统的反应。单壁碳纳米管处理的EC具有更高的抗氧化水平，如POD，CAT和GSH比对照组EC。对照组EC主要依靠AsA-GSH和GPX循环吸收H_2O_2，而添加SWCNTs组EC主要依靠CAT，导致H_2O_2和MDA含量降低。通过添加单壁碳纳米管提高脱水中的抗氧化剂水平，增强了细胞在冻存过程中对损伤的抵抗力。加入SWCNT的冻存EC的ROS信号平衡稳定。单壁碳纳米管在冻存过程中调节了EC的氧化应激反应，并通过维持ROS的稳态来控制氧化损伤，以获得冻存后的高存活率。本研究首次系统阐述了碳纳米材料在植物氧化胁迫反应中的调控作用，为纳米材料在低温生物学领域的应用提供了新的思路。
Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field. This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during the dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect the ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT, and GSH than the control group EC. The EC mainly depended on the AsA-GSH and GPX cycle to scavenge H2O2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H2O2 and MDA. The elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation. The SWCNTs regulated oxidative stress responses of EC during the process and controlled oxidative damages by the maintenance of ROS homeostasis to achieve a high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology.
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影响因子：
7.2
作者：
Dat, JF;Pellinen, R;Van Breusegem, F
通讯作者：
Van Breusegem, F
影响因子：
3
作者：
Decruse, SW;Seeni, S;Nair, GM
通讯作者：
Nair, GM
DOI：
10.1201/9780203647073.ch6
发表时间：
2004-01-01
期刊：
LIFE IN THE FROZEN STATE
影响因子：
--
作者：
Benson, EE;Bremner, D
通讯作者：
Bremner, D
影响因子：
2.6
作者：
Baskaran, Ponnusamy;Chukwujekwu, Jude C.;Van Staden, Johannes
通讯作者：
Van Staden, Johannes
影响因子：
--
作者：
Aslani F;Bagheri S;Muhd Julkapli N;Juraimi AS;Hashemi FS;Baghdadi A
通讯作者：
Baghdadi A