Echocardiography protocol: A tool for infrequently used parameters in mice.

Echocardiography protocol: A tool for infrequently used parameters in mice.
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DOI:
10.3389/fcvm.2022.1038385
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发表时间:
2022
影响因子:
3.6
通讯作者:
Shehadeh, Lina A.
Shehadeh, Lina A.
中科院分区:
医学3区
文献类型:
--
作者:
Todd, Emily Ann;Williams, Monique;Kamiar, Ali;Rasmussen, Monica Anne;Shehadeh, Lina A.

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超声心动图常用于评价心血管疾病啮齿动物模型的心脏功能。尽管在已发表的协议或手册中详细描述了获取常用超声心动图参数的方法,但许多重要参数定义不清,并且通常对主观解释开放。这种缺乏统一性的情况导致了对已发表文献中相同参数的相互矛盾的解释。特别是,在更罕见的小鼠模型中定义更深奥的病因所需的参数,如二尖瓣返流、二尖瓣狭窄、肺动脉返流和主动脉瓣返流,通常仍然不明确。本文旨在为不常用的超声心动图参数的获取和解释提供实用指南,并为右心室(RV)、肺动脉(PA)、肺动脉瓣(PV)、左心房(LA)、二尖瓣(MV)和主动脉瓣(AoV)结构和功能的综合分析提供框架。
Echocardiography is frequently used to evaluate cardiac function in rodent models of cardiovascular disease. Whereas methods to acquire the commonly used echocardiography parameters are well-described in published protocols or manuals, many important parameters are ill-defined and often open to subjective interpretation. Such lack of uniformity has engendered conflicting interpretations of the same parameters in published literature. In particular, parameters such as mitral regurgitation, mitral stenosis, pulmonary regurgitation, and aortic regurgitation that are required to define more esoteric etiologies in rarer mouse models often remain equivocal. The aim of this methods paper is to provide a practical guide to the acquisition and interpretation of infrequently used echocardiography parameters and set a framework for comprehensive analyses of right ventricle (RV), pulmonary artery (PA) pulmonary valve (PV), left atrium (LA), mitral valve (MV), and aortic valve (AoV) structure and function.
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