Preharvest Methyl Jasmonate Treatment Increased Glucosinolate Biosynthesis, Sulforaphane Accumulation, and Antioxidant Activity of Broccoli.
Preharvest Methyl Jasmonate Treatment Increased Glucosinolate Biosynthesis, Sulforaphane Accumulation, and Antioxidant Activity of Broccoli.
复制标题
采前茉莉酸甲酯处理增加了西兰花的芥子油苷生物合成、萝卜硫素积累和抗氧化活性
DOI：
10.3390/antiox11071298
复制
发表时间：
2022-06-29
期刊：
影响因子：
--
通讯作者：
Wu Q
中科院分区：
文献类型：
--
作者：
Wang J;Mao S;Liang M;Zhang W;Chen F;Huang K;Wu Q
西兰花由于其营养价值和丰富的生物活性化合物，如芥子油苷（GSL）和水解产物，成为植物防御，癌症预防和人类更高的抗氧化活性的次级代谢产物，越来越受消费者欢迎。本研究采用40 μmol/L茉莉酸甲酯（MeJA）对青花菜进行喷雾处理。以收获的青花菜小花、茎和叶为材料，测定了GSL、萝卜硫素、总酚和黄酮的含量，以及黑芥子酶活性、抗氧化活性和GSL生物合成相关基因的表达。总体结果显示，GSL生物合成和萝卜硫素积累最有可能是由外源MeJA处理通过上调CYP 83 A1、SUR 1、UGT 74 B1和SOT 18基因的表达诱导的。外源MeJA处理对低GSL含量的青花菜品种（燕秀）GSL合成的促进作用比高GSL含量的青花菜品种（湘绿3号）更显著。MeJA处理对青花菜小花的促长作用比茎和叶更显著。有趣的是，MeJA处理后，西兰花小花中总黄酮含量大幅增加，但总酚类物质没有。同样，2，2-二苯基-1-苦基肼（DPPH）自由基清除能力，trolox等效抗氧化能力（ABTS），铁还原抗氧化能力（FRAP）在西兰花小花MeJA处理后更高。综上所述，茉莉酸甲酯介导了活性物质的代谢，促进了GSL的合成、萝卜硫素和黄酮类化合物的积累，与诱导西兰花小花抗氧化活性呈正相关。因此，采前添加40 μM的MeJA是提高青花菜花营养价值的有效途径。
Broccoli is becoming increasingly popular among consumers owing to its nutritional value and rich bioactive compounds, such glucosinolates (GSLs) and hydrolysis products, which are secondary metabolites for plant defense, cancer prevention, and higher antioxidant activity for humans. In this study, 40 μmol/L methyl jasmonate (MeJA) was sprayed onto broccoli from budding until harvest. The harvested broccoli florets, stem, and leaves were used to measure the contents of GSLs, sulforaphane, total phenolics, and flavonoids, as well as myrosinase activity, antioxidant activity, and gene expression involved in GSL biosynthesis. The overall results revealed that GSL biosynthesis and sulforaphane accumulation were most likely induced by exogenous MeJA treatment by upregulating the expression of CYP83A1, SUR1, UGT74B1, and SOT18 genes. Exogenous MeJA treatment more remarkably contributed to the increased GSL biosynthesis in broccoli cultivars with low-level GSL content (Yanxiu) than that with high-level GSLs (Xianglv No.3). Moreover, MeJA treatment had a more remarkable increasing effect in broccoli florets than stem and leaves. Interestingly, total flavonoid content substantially increased in broccoli florets after MeJA treatment, but total phenolics did not. Similarly, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, trolox-equivalent antioxidant capacity (ABTS), and ferric-reducing antioxidant power (FRAP) were higher in broccoli floret after MeJA treatment. In conclusion, MeJA mediated bioactive compound metabolism, had positive effects on GSL biosynthesis, sulforaphane, and flavonoids accumulation, and showed positive correlation on inducing higher antioxidant activities in broccoli floret. Hence, preharvest supplementation with 40 μM MeJA could be a good way to improve the nutritional value of broccoli florets.
登录
查看更多内容
影响因子：
2.1
作者：
Huang, K.;Lin, J. C.;Xiao, W. J.
通讯作者：
Xiao, W. J.
影响因子：
5.6
作者：
Chiu YC;Juvik JA;Ku KM
通讯作者：
Ku KM
DOI：
10.21273/jashs.127.5.807
发表时间：
2002-09-01
影响因子：
1.9
作者：
Brown, AF;Yousef, GG;Juvik, JA
通讯作者：
Juvik, JA
影响因子：
4.3
作者：
Guo, Rongfang;Yuan, Gaofeng;Wang, Qiaomei
通讯作者：
Wang, Qiaomei
影响因子：
3.9
作者：
Dominguez-Perles, Raul;Carmen Martinez-Ballesta, Maria;Moreno, Diego A.
通讯作者：
Moreno, Diego A.