Targeted Metabolomic and Transcriptomic Analyses of "Red Russian" Kale (Brassicae napus var. pabularia) Following Methyl Jasmonate Treatment and Larval Infestation by the Cabbage Looper (Trichoplusia ni Hübner).
Targeted Metabolomic and Transcriptomic Analyses of "Red Russian" Kale (Brassicae napus var. pabularia) Following Methyl Jasmonate Treatment and Larval Infestation by the Cabbage Looper (Trichoplusia ni Hübner).
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DOI：
10.3390/ijms19041058
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发表时间：
2018-04-02
影响因子：
5.6
通讯作者：
Ku KM
中科院分区：
文献类型：
--
作者：
Chiu YC;Juvik JA;Ku KM
茉莉酸甲酯（MeJA）是茉莉酸（JA）途径中合成的一种代谢产物，可上调油菜科植物芥子油苷（GS）的生物合成。外源应用茉莉酸甲酯已被证明增加组织GS浓度和黑芥子酶介导的GS水解产物（GSHPs）的形成。体外和体内试验已经证明了某些GSH的潜在健康促进作用。MeJA还已知在芸苔属物种中引发和诱导与昆虫植食性防御机制相关的基因。为了研究茉莉酸甲酯（MeJA）诱导的甘蓝GS合成与昆虫防御的关系，以“俄罗斯红”甘蓝为试材，研究了3种处理对甘蓝GS合成的影响。pabularia）幼苗：（1）250 µM MeJA叶喷雾处理;（2）用甘蓝夜蛾（粉纹夜蛾（Trichoplusia ni（Hübner）幼虫进行叶侵染;（3）对照处理（既不进行幼虫侵染，也不施用MeJA）。从三个处理的叶组织样品，然后测定GS和GSHP浓度的变化，GS基因的生物合成表达，黑芥子酶活性。GS积累水平和GS基因表达水平在三个处理之间存在较大差异。结果表明，受虫害的样品脂肪族GS积累量显著降低，而MeJA和T。Ni侵染处理诱导了吲哚GS的大量积累。与对照组相比，用MeJA和昆虫处理的样品中CYP 81 F4、MYB 34和MYB 122的基因表达水平显著上调，这解释了吲哚GS浓度的增加。结果表明，MeJA的应用和昆虫植食反应促进的代谢变化有共同的诱导机制。这项工作提供了潜在的有用信息羽衣甘蓝害虫防治和营养品质。
Methyl jasmonate (MeJA), synthesized in the jasmonic acid (JA) pathway, has been found to upregulate glucosinolate (GS) biosynthesis in plant species of the Brassicaceae family. Exogenous application of MeJA has shown to increase tissue GS concentrations and the formation of myrosinase-mediated GS hydrolysis products (GSHPs). In vitro and in vivo assays have demonstrated the potential health-promoting effects of certain GSHPs. MeJA is also known to elicit and induce genes associated with defense mechanisms to insect herbivory in Brassica species. To investigate the relationship between MeJA-induced GS biosynthesis and insect defense, three treatments were applied to “Red Russian” kale (Brassicae napus var. pabularia) seedlings: (1) a 250 µM MeJA leaf spray treatment; (2) leaf infestation with larvae of the cabbage looper (Trichoplusia ni (Hübner)); (3) control treatment (neither larval infestation nor MeJA application). Samples of leaf tissue from the three treatments were then assayed for changes in GS and GSHP concentrations, GS gene biosynthesis expression, and myrosinase activity. Major differences were observed between the three treatments in the levels of GS accumulation and GS gene expression. The insect-damaged samples showed significantly lower aliphatic GS accumulation, while both MeJA and T. ni infestation treatments induced greater accumulation of indolyl GS. The gene expression levels of CYP81F4, MYB34, and MYB122 were significantly upregulated in samples treated with MeJA and insects compared to the control group, which explained the increased indolyl GS concentration. The results suggest that the metabolic changes promoted by MeJA application and the insect herbivory response share common mechanisms of induction. This work provides potentially useful information for kale pest control and nutritional quality.
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影响因子：
6.1
作者：
Hahn, Christoph;Mueller, Anja;Albach, Dirk
通讯作者：
Albach, Dirk
影响因子：
3.7
作者：
Badenes-Perez FR;Gershenzon J;Heckel DG
通讯作者：
Heckel DG
影响因子：
2.7
作者：
Bidart-Bouzat, M. Gabriela;Kliebenstein, Daniel
通讯作者：
Kliebenstein, Daniel
DOI：
10.21273/jashs.127.5.807
发表时间：
2002-09-01
影响因子：
1.9
作者：
Brown, AF;Yousef, GG;Juvik, JA
通讯作者：
Juvik, JA
影响因子：
6.9
作者：
Augustine R;Majee M;Gershenzon J;Bisht NC
通讯作者：
Bisht NC