Acute toxicity and gastroprotective role of M. pruriens in ethanol-induced gastric mucosal injuries in rats.
Acute toxicity and gastroprotective role of M. pruriens in ethanol-induced gastric mucosal injuries in rats.
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DOI：
10.1155/2013/974185
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发表时间：
2013
影响因子：
--
通讯作者：
Abdulla MA
中科院分区：
文献类型：
--
作者：
Golbabapour S;Hajrezaie M;Hassandarvish P;Abdul Majid N;Hadi AH;Nordin N;Abdulla MA
本研究旨在评价羊栖菜叶醇提物对大鼠乙醇性胃粘膜损伤的保护作用。将48只大鼠随机分为8组：阴性对照组、提取物对照组、溃疡对照组、参比对照组和4个实验组。空白对照组和溃疡对照组口服羧甲基纤维素(CMC)作为治疗前处理。参照对照为口服奥美拉唑(20 mg/kg)。分别给提取物对照组(5 0 0 mg/kg)、实验组(6 2.5、12 5、2 5 0、5 0 0 mg/kg)灌胃紫云英叶乙醇提取物。1 h后，阴性对照组和提取物对照组灌胃给予CMC。其他组接受的是无水乙醇。1 h后处死大鼠，溃疡对照组大鼠胃粘膜损伤明显，胃壁粘液减少，胃粘膜损伤严重。该提取物可使Hsp70蛋白表达上调，Bax蛋白表达下调，胃腺部对高碘酸席夫摄取作用增强。胃粘膜匀浆显示出明显的抗氧化性能，PGE2合成增加，而丙二醛显著降低。羊栖菜叶的乙醇提取物无毒(~lt；5 g/kg)，并能增强对出血性粘膜损伤的防御机制。
The investigation was to evaluate gastroprotective effects of ethanolic extract of M. pruriens leaves on ethanol-induced gastric mucosal injuries in rats. Forty-eight rats were divided into 8 groups: negative control, extract control, ulcer control, reference control, and four experimental groups. As a pretreatment, the negative control and the ulcer control groups were orally administered carboxymethylcellulose (CMC). The reference control was administered omeprazole orally (20 mg/kg). The ethanolic extract of M. pruriens leaves was given orally to the extract control group (500 mg/kg) and the experimental groups (62.5, 125, 250, and 500 mg/kg). After 1 h, CMC was given orally to the negative and the extract control groups. The other groups received absolute ethanol. The rats were sacrificed after 1 h. The ulcer control group exhibited significant mucosal injuries with decreased gastric wall mucus and severe damage to the gastric mucosa. The extract caused upregulation of Hsp70 protein, downregulation of Bax protein, and intense periodic acid schiff uptake of glandular portion of stomach. Gastric mucosal homogenate showed significant antioxidant properties with increase in synthesis of PGE2, while MDA was significantly decreased. The ethanolic extract of M. pruriens leaves was nontoxic (<5 g/kg) and could enhance defensive mechanisms against hemorrhagic mucosal lesions.
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DOI：
10.1179/174329211x12968219310756
发表时间：
2011
期刊：
Redox report : communications in free radical research
影响因子：
--
作者：
Handa O;Naito Y;Yoshikawa T
通讯作者：
Yoshikawa T
影响因子：
6.7
作者：
CLAMP, JR;ALLEN, A;ROBERTS, GP
通讯作者：
ROBERTS, GP
影响因子：
2.4
作者：
Dursun H;Bilici M;Albayrak F;Ozturk C;Saglam MB;Alp HH;Suleyman H
通讯作者：
Suleyman H
影响因子：
3.4
作者：
Johnston, CJ;Williams, JP;Finkelstein, JN
通讯作者：
Finkelstein, JN
DOI：
10.2174/1566524013363483
发表时间：
2001-09-01
期刊：
Current Molecular Medicine (Hilversum)
影响因子：
--
作者：
Bandyopadhyay, Debashis;Biswas, Kaushik;Banerjee, Ranajit K.
通讯作者：
Banerjee, Ranajit K.