An in vitro study on the effect of free amino acids alone or in combination with nisin on biofilms as well as on planktonic bacteria of Streptococcus mutans.
An in vitro study on the effect of free amino acids alone or in combination with nisin on biofilms as well as on planktonic bacteria of Streptococcus mutans.
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单独使用游离氨基酸或与乳链菌肽联合使用对变形链球菌生物膜以及浮游细菌影响的体外研究
DOI：
10.1371/journal.pone.0099513
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Deng D
中科院分区：
文献类型：
--
作者：
Tong Z;Zhang L;Ling J;Jian Y;Huang L;Deng D
Free D-amino acids (D-AAs) are one of the most striking features of the peptidoglycan composition in bacteria and play a key role in regulating and disassembling bacterial biofilms. Previous studies have indicated that the antimicrobial peptide nisin can inhibit the growth of the cariogenic bacteria Streptococcus mutans. The present study investigated the effect of free amino acids either alone or in combination with nisin on biofilm and on planktonic S. mutans bacteria. The results of the MIC and MBC analyses showed that D-cysteine (Cys), D- or L-aspartic acid (Asp), and D- or L-glutamic acid (Glu) significantly improve the antibacterial activity of nisin against S. mutans and that the mixture of D-Cys, D-Asp, and D-Glu (3D-AAs) and the mixture of L-Cys, L-Asp, and L-Glu (3L-AAs) at a concentration of 40 mM can prevent S. mutans growth. Crystal violet staining showed that the D- or L-enantiomers of Cys, Asp, and Glu at a concentration of 40 mM can inhibit the formation of S. mutans biofilms, and their mixture generated a stronger inhibition than the components alone. Furthermore, the mixture of the three D-AAs or L-AAs may improve the antibacterial activity of nisin against S. mutans biofilms. This study underscores the potential of free amino acids for the enhancement of the antibacterial activity of nisin and the inhibition of the cariogenic bacteria S. mutans and biofilms.
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影响因子：
8
作者：
Cava, Felipe;Lam, Hubert;de Pedro, Miguel A.;Waldor, Matthew K.
通讯作者：
Waldor, Matthew K.
影响因子：
3
作者：
Tong, Zhongchun;Dong, Liping;Ni, Longxing
通讯作者：
Ni, Longxing
影响因子：
14
作者：
Sanchez, Carlos J., Jr.;Prieto, Edna M.;Wenke, Joseph C.
通讯作者：
Wenke, Joseph C.
影响因子：
2.9
作者：
Hasper, HE;de Kruijff, B;Breukink, E
通讯作者：
Breukink, E
影响因子：
11.4
作者：
Cava, Felipe;de Pedro, Miguel A.;Waldor, Matthew K.
通讯作者：
Waldor, Matthew K.