Expressed sequence tags of the peanut pod nematode Ditylenchus africanus: the first transcriptome analysis of an Anguinid nematode.
Expressed sequence tags of the peanut pod nematode Ditylenchus africanus: the first transcriptome analysis of an Anguinid nematode.
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DOI：
10.1016/j.molbiopara.2009.04.004
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发表时间：
2009-09
影响因子：
1.5
通讯作者：
Gheysen, Godelieve
中科院分区：
文献类型：
--
作者：
Haegeman, Annelies;Jacob, Joachim;Vanholme, Bartel;Kyndt, Tina;Mitreva, Makedonka;Gheysen, Godelieve
在这项研究中，4847表达测序标签（EST）的迁移植物寄生线虫非洲茎线虫（花生豆荚线虫）的混合阶段进行了调查。这是第一个属于Anguinidae（Rhabditida，Sphaerularioidea总科）的线虫的分子调查。这些序列被聚类为2596个unigenes，其中43%与已知的蛋白质、核苷酸、线虫EST或植物寄生线虫基因组序列没有任何同源性。基因本体图显示，大多数推定的蛋白质参与发育和生殖过程。此外，还鉴定了参与氧化应激和脱水作用的单基因，如莱亚（晚期胚胎发生丰富蛋白）和海藻糖-6-磷酸合酶。其他标签显示与先前描述的参与寄生的基因（扩展蛋白、SEC-2、钙网蛋白、14-3-3b和各种过敏原蛋白）同源。原位杂交结果表明，一个假定的扩展蛋白和毒液过敏原蛋白的表达被限制在线虫的腺细胞区域，与它们在寄生中的假定作用一致。此外，7个推定的新的候选寄生基因的基础上预测的信号肽在相应的蛋白质序列和同源EST专属于寄生线虫。这些基因对于进一步的研究和功能鉴定是有趣的。最后，34个unigenes被保留作为未来RNAi实验的良好靶候选物，因为它们的线虫特异性和观察到的秀丽隐杆线虫同源物的致死表型。
In this study, 4847 expressed sequenced tags (ESTs) from mixed stages of the migratory plant-parasitic nematode Ditylenchus africanus (peanut pod nematode) were investigated. It is the first molecular survey of a nematode which belongs to the family of the Anguinidae (order Rhabditida, superfamily Sphaerularioidea). The sequences were clustered into 2596 unigenes, of which 43% did not show any homology to known protein, nucleotide, nematode EST or plant-parasitic nematode genome sequences. Gene ontology mapping revealed that most putative proteins are involved in developmental and reproductive processes. In addition unigenes involved in oxidative stress as well as in anhydrobiosis, such as LEA (late embryogenesis abundant protein) and trehalose-6-phosphate synthase were identified. Other tags showed homology to genes previously described as being involved in parasitism (expansin, SEC-2, calreticulin, 14-3-3b and various allergen proteins). In situ hybridization revealed that the expression of a putative expansin and a venom allergen protein was restricted to the gland cell area of the nematode, being in agreement with their presumed role in parasitism. Furthermore, 7 putative novel candidate parasitism genes were identified based on the prediction of a signal peptide in the corresponding protein sequence and homologous ESTs exclusively in parasitic nematodes. These genes are interesting for further research and functional characterization. Finally, 34 unigenes were retained as good target candidates for future RNAi experiments, because of their nematode specific nature and observed lethal phenotypes of Caenorhabditis elegans homologs.
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影响因子：
1.2
作者：
Jones, JT;Furlanetto, C;Kikuchi, T
通讯作者：
Kikuchi, T
微阵列和 EST 数据库对 mRNA 表达水平的估计不同：：C-elegans 的蛋白质长度与表达曲线--：第 30 条。 30
影响因子：
4.4
作者：
Munoz, ET;Bogarad, LD;Deem, MW
通讯作者：
Deem, MW
影响因子：
4.9
作者：
Haegeman, Annelies;Vanholme, Bartel;Gheysen, Godelieve
通讯作者：
Gheysen, Godelieve
影响因子：
14.9
作者：
Min XJ;Butler G;Storms R;Tsang A
通讯作者：
Tsang A
影响因子：
3.5
作者：
Jaubert, S;Milac, AL;Rosso, MN
通讯作者：
Rosso, MN