Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy.
Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy.
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DOI：
10.1021/ja210081h
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发表时间：
2012-02-29
影响因子：
15
通讯作者：
Xie, Xiaoliang Sunney
中科院分区：
文献类型：
--
作者：
Fu, Dan;Lu, Fa-Ke;Zhang, Xu;Freudiger, Christian;Pernik, Douglas R.;Holtom, Gary;Xie, Xiaoliang Sunney
受激拉曼散射(SRS)显微镜是一种新发展起来的无标记化学成像技术，它克服了共焦拉曼显微镜的速度限制，同时避免了相干反斯托克斯拉曼散射(CARS)显微镜的非共振背景问题。以前的演示仅限于单拉曼波段测量。我们提出了一种新的调制复用方法，该方法允许使用快速傅立叶变换来实时检测多个物种。我们演示了三元混合物化学浓度的定量测定。此外，我们还探索了两种成像应用：(1)定量测定微藻培养物中的油含量以及色素和蛋白质浓度；(2)3D高分辨率成像，用于体外小鼠皮肤组织中血液、血脂和蛋白质的分布。我们相信，定量多重SRS独一无二地结合了快速无标记成像的优势和拉曼光谱的指纹识别能力，使其能够在脂质生物学和生物医学成像中得到广泛应用。
Stimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman while avoiding the nonresonant-background problem of coherent anti-Stokes Raman scattering (CARS) microscopy. Previous demonstrations were limited to single Raman band measurement. We present a novel modulation multiplexing approach that allows real-time detection of multiple species using the fast Fourier-transform. We demonstrate quantitative determination of chemical concentration of a ternary mixture. Furthermore, two imaging applications are pursued: (1) quantitative determination of oil content, as well as pigment and protein concentration in microalgae cultures; (2) 3D high resolution imaging of blood, lipids, and protein distribution in ex vivo mouse skin tissue. We believe quantitative multiplex SRS uniquely combines the advantage of fast label-free imaging with the fingerprinting capability of Raman spectroscopy and enables numerous applications lipid biology as well as biomedical imaging.
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影响因子：
48
作者：
Wang, Meng C.;Min, Wei;Freudiger, Christian W.;Ruvkun, Gary;Xie, X. Sunney
通讯作者：
Xie, X. Sunney
影响因子：
3.3
作者：
Nandakumar, P.;Kovalev, A.;Volkmer, A.
通讯作者：
Volkmer, A.
利用多重 CARS 显微镜成像脂质膜的热力学状态
影响因子：
3.3
作者：
Müller, M;Schins, JM
通讯作者：
Schins, JM
影响因子：
35
作者：
通讯作者：
--
影响因子：
4
作者：
Kano, H;Hamaguchi, H
通讯作者：
Hamaguchi, H