Characterisation of the pathogenicity of strains of Pseudomonas syringae towards cherry and plum
Characterisation of the pathogenicity of strains of Pseudomonas syringae towards cherry and plum
复制标题
丁香假单胞菌菌株对樱桃和李子的致病性特征
DOI：
10.1101/227223
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hulin M
中科院分区：
文献类型：
--
作者：
Hulin M
细菌性溃疡病是樱桃、李等核果类果树的主要病害。它是由铜绿假单胞菌属种复合体中的致病变种引起的，包括铜绿假单胞菌（Psm）生理小种1（R1）、Psm生理小种2（R2）和铜绿假单胞菌（Pss）。Psm R1和Psm R2最初被指定为相同的致病变种;然而，系统发育分析显示它们是远缘的，分别落入第3和第1组。本研究在田间和实验室条件下，对18个新基因组测序的樱桃和李疫霉菌株的致病性进行了表征。田间试验证实，樱桃品种默顿荣耀表现出广泛的所有分支的电阻。Psm R1含有对樱桃和李具有不同特异性的菌株。检查了易处理的基于实验室的测定在整个树上再现评估的能力。使用切枝或叶的测定实现了良好的相关性，虽然只有切枝测定能够可靠地区分在田间看到的品种差异。测定离体叶片中的细菌增殖可区分病原体和非病原体，因此适用于常规检测。在樱桃叶片中，症状外观区分Psm种族从非病原体，这引发了过敏反应。Pss的致病菌株在所有组织中迅速诱导疾病病变，并表现出比半活体营养型Psm更坏死的生活方式。这种对病原体相互作用的深入研究，宿主抗性的鉴定和实验室检测的优化为未来对溃疡病中宿主-病原体相互作用的遗传解剖提供了框架。
Bacterial canker is a major disease ofPrunus avium(cherry),Prunus domestica(plum) and other stone fruits. It is caused by pathovars within thePseudomonas syringaespecies complex includingP. syringaepv.morsprunorum(Psm) race 1 (R1), Psm race 2 (R2) andP. syringaepv.syringae(Pss). Psm R1 and Psm R2 were originally designated as the same pathovar; however, phylogenetic analysis revealed them to be distantly related, falling into phylogroups 3 and 1, respectively. This study characterized the pathogenicity of 18 newly genome‐sequencedP. syringaestrains on cherry and plum, in the field and laboratory. The field experiment confirmed that the cherry cultivar Merton Glory exhibited a broad resistance to all clades. Psm R1 contained strains with differential specificity on cherry and plum. The ability of tractable laboratory‐based assays to reproduce assessments on whole trees was examined. Good correlations were achieved with assays using cut shoots or leaves, although only the cut shoot assay was able to reliably discriminate cultivar differences seen in the field. Measuring bacterial multiplication in detached leaves differentiated pathogens from nonpathogens and was therefore suitable for routine testing. In cherry leaves, symptom appearance discriminated Psm races from nonpathogens, which triggered a hypersensitive reaction. Pathogenic strains of Pss rapidly induced disease lesions in all tissues and exhibited a more necrotrophic lifestyle than hemibiotrophic Psm. This in‐depth study of pathogenic interactions, identification of host resistance and optimization of laboratory assays provides a framework for future genetic dissection of host–pathogen interactions in the canker disease.
登录
查看更多内容
樱桃在田间和实验室对细菌性溃疡病（丁香假单胞菌丁香致病变种）的敏感性
DOI：
--
发表时间：
2016
期刊：
影响因子：
--
作者：
S. Farhadfar;M. Keshavarzi;N. Bouzari;L. Moghadam;A. Soleimani
通讯作者：
A. Soleimani
俄勒冈州用于腌制、冷冻和罐装的甜樱桃品种
DOI：
--
发表时间：
2013
期刊：
影响因子：
--
作者：
L. Long;J. Olsen
通讯作者：
J. Olsen
影响因子：
2.7
作者：
S. Soylu;I. Brown;J. Mansfield
通讯作者：
S. Soylu;I. Brown;J. Mansfield
核果细菌性溃疡病：Pseudomonas mors-prunorum 和 P. syringae 的感染实验
DOI：
--
发表时间：
1966
期刊：
影响因子：
--
作者：
J. Crosse;C. Garrett
通讯作者：
C. Garrett
使用rep-PCR 鉴别甜樱桃和野樱桃中的丁香假单胞菌分离株
影响因子：
1.8
作者：
J. Vicente;S. J. Roberts
通讯作者：
S. J. Roberts