Elevated production of the aromatic fragrance molecule, 2-phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes.
Elevated production of the aromatic fragrance molecule, 2-phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes.
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DOI：
10.1002/jctb.5597
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发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Chuck C
中科院分区：
文献类型：
--
作者：
Chantasuban T;Santomauro F;Gore-Lloyd D;Parsons S;Henk D;Scott RJ;Chuck C
2-苯基乙醇（2 PE）是一种主要用于香水和食品工业的香料分子。它可以由石油化工产品廉价地制成，然而，这不适合大多数食品应用。目前，生物衍生化合物的主要生产方法是提取玫瑰花瓣中的微量成分，这是非常昂贵的。潜在的发酵可以提供一种廉价的，天然来源的替代品。在该研究中，从酵母Metschnikowia pulcherrima生产2 PE，在扩大到2L分批和连续操作之前在烧瓶中进行优化。2 PE可以在从头工艺条件下以高滴度生产，在2 L搅拌生物反应器中达到高达1500 mg L−1。这是迄今为止报道的最高从头滴度，并且通过高糖负荷加上低氮条件实现。该方法也成功地以连续模式运行，保持650 mg L-1的2 PE浓度。通过苯丙氨酸的从头转化和从上清液中半连续固相萃取进一步增加2 PE产量。在最佳条件下，2 PE的产量为14000 mg L-1。这里提出的工作提供了一种新的途径，通过可扩展的发酵，利用非常适合工业生物技术的强大酵母，天然来源的2 PE。版权所有© 2018作者. Journal of Chemical Technology & Biotechnology由John Wiley & Sons Ltd代表化学工业协会出版。
2‐phenylethanol (2PE) is a fragrance molecule predominantly used in perfumes and the food industry. It can be made from petrochemicals inexpensively, however, this is unsuitable for most food applications. Currently, the main method of production for the bio‐derived compound is to extract the trace amounts found in rose petals, which is extremely costly. Potentially fermentation could provide an inexpensive, naturally sourced, alternative. In this investigation, 2PE was produced from the yeast Metschnikowia pulcherrima, optimised in flasks before scaling to 2 L batch and continuous operation. 2PE can be produced in high titres under de novo process conditions with up to 1500 mg L−1 achieved in a 2 L stirred bioreactor. This is the highest reported de novo titre to date, and achieved through high sugar loadings coupled with low nitrogen conditions. The process successfully ran in continuous mode also, with a concentration of 650 mg L−1 of 2PE being maintained. The 2PE production was further increased by the ex novo conversion of phenylalanine and semi‐continuous solid phase extraction from the supernatant. Under optimal conditions 14 000 mg L−1 of 2PE was produced. The work presented here offers a novel route to naturally sourced 2PE through a scalable fermentation with a robust yeast highly suited to industrial biotechnology. © 2018 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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DOI：
10.1016/j.cep.2013.08.008
发表时间：
2013-10-01
影响因子：
4.3
作者：
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通讯作者：
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影响因子：
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发表时间：
1973-01-01
期刊：
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影响因子：
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影响因子：
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DOI：
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发表时间：
2010-03-09
影响因子：
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