Neutrophil transit time and localization within the megakaryocyte define morphologically distinct forms of emperipolesis.
Neutrophil transit time and localization within the megakaryocyte define morphologically distinct forms of emperipolesis.
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DOI：
10.1182/bloodadvances.2021005097
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发表时间：
2022-04-12
期刊：
影响因子：
7.5
通讯作者：
Nigrovic, Peter A.
中科院分区：
文献类型：
--
作者：
Huang, Frank Y.;Cunin, Pierre;Radtke, Felix A.;Darbousset, Roxane;Grieshaber-Bouyer, Ricardo;Nigrovic, Peter A.
Emperipolesis（中性粒细胞通过巨核细胞）发生在快速和缓慢的形式，形态不同。巨核细胞内的中性粒细胞位于核小体和细胞质中，靠近分界膜系统、内质网和细胞核。中性粒细胞在一个称为empipolesis的过程中通过巨核细胞，但目前尚不清楚这种相互作用是单一类型的细胞间相互作用还是一组不同的过程。使用小鼠在体外模型，我们的特点empipiopolesis活细胞旋转盘显微镜和电子显微镜。大约有一半的中性粒细胞迅速离开巨核细胞，通常在10分钟或更短的时间内，当它们通过宿主细胞时显示出阿米巴样形态（快速empipolesis）。剩余的中性粒细胞呈现无柄形态，大多数在巨核细胞内至少保留60分钟（缓慢的emperipolesis）。这些中性粒细胞通常位于巨核细胞核附近。通过超微结构评估，所有内化的中性粒细胞在形态上保持完整。大多数中性粒细胞位于帝国体内，但也有一些可以看到离开帝国体进入细胞质。胞浆中的中性粒细胞与血小板形成分界膜系统或核周内质网紧密接触。这些结果表明，巨核细胞的emperipolesis反映了至少2个不同的过程，不同的过境时间和形态，快速和缓慢emperipolesis，表明不同的生理功能。
Emperipolesis (neutrophil transit through megakaryocytes) occurs in fast and slow forms that differ morphologically. Intramegakaryocytic neutrophils reside in emperisomes and in cytoplasm near the demarcation membrane system, endoplasmic reticulum, and nucleus. Neutrophils transit through megakaryocytes in a process termed emperipolesis, but it is unknown whether this interaction is a single type of cell-in-cell interaction or a set of distinct processes. Using a murine in vitro model, we characterized emperipolesis by live-cell spinning disk microscopy and electron microscopy. Approximately half of neutrophils exited the megakaryocyte rapidly, typically in 10 minutes or less, displaying ameboid morphology as they passed through the host cell (fast emperipolesis). The remaining neutrophils assumed a sessile morphology, most remaining within the megakaryocyte for at least 60 minutes (slow emperipolesis). These neutrophils typically localized near the megakaryocyte nucleus. By ultrastructural assessment, all internalized neutrophils remained morphologically intact. Most neutrophils resided within emperisomes, but some could be visualized exiting the emperisome to enter the cell cytoplasm. Neutrophils in the cytoplasm assumed close contact with the platelet-forming demarcation membrane system or the perinuclear endoplasmic reticulum. These findings reveal that megakaryocyte emperipolesis reflects at least 2 distinct processes differing in transit time and morphology, fast and slow emperipolesis, suggesting divergent physiologic functions.
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DOI：
10.1083/jcb.201304054
发表时间：
2013-06-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Machlus KR;Italiano JE Jr
通讯作者：
Italiano JE Jr
影响因子：
20.3
作者：
Beaulieu, Lea M.;Lin, Elaine;Freedman, Jane E.
通讯作者：
Freedman, Jane E.
影响因子：
20.3
作者：
Centurione, L;Di Baldassarre, A;Migliaccio, AR
通讯作者：
Migliaccio, AR
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
7.7
作者：
Cunin, Pierre;Bouslama, Rim;Nigrovic, Peter A.
通讯作者：
Nigrovic, Peter A.