The effects of the alkyl polycyclic aromatic hydrocarbon retene on rainbow trout (Oncorhynchus mykiss) immune response.
The effects of the alkyl polycyclic aromatic hydrocarbon retene on rainbow trout (Oncorhynchus mykiss) immune response.
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烷基多环芳烃视黄醇对虹鳟鱼（Oncorhynchus mykiss）免疫反应的影响
DOI：
10.1016/j.aquatox.2010.07.020
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发表时间：
2010
影响因子：
4.5
通讯作者：
van den Heuvel M.R.
中科院分区：
文献类型：
--
作者：
Hogan N.S;Lee K.S;Köllner B;van den Heuvel M.R.
研究了烷基多环芳烃（PAH）retene（7-异丙基-1-甲基菲）对虹鳟鱼的免疫毒性。Retene是一种常见的烷基PAH，与陆生植物或工业废水的燃烧有关。分别以0、1、10 mg/kg的剂量对虹鳟鱼进行单次腹腔注射，于21日龄取样。在每个retene治疗，共注射福尔马林杀死杀鲑气单胞菌或磷酸盐缓冲盐水假刺激鳟鱼免疫系统。在最高剂量（10 mg/kg）时，血液白细胞总数增加，但仅A。杀鲑素注射组。这一结果被血细胞分类计数中白细胞的增加所掩盖。A整体上涨。由于抗原注射，杀沙门氏菌特异性抗体滴度降低，并且在10 mg/kg retene剂量下也存在抗体产生应答的显著刺激。在组织水平上，免疫组织化学显示，在最高剂量的retene下，脾脏和头肾中的B淋巴细胞密度更大。用定量RT-PCR方法检测了脾脏和头肾中Th 2型标志物CD 4、TCR、MHCII、TNFα和Th 1型标志物CD 8、INFγ的表达。A.杀鲑抗原对任一组织中Th 1相关基因表达的影响，但在两种retene浓度下，在脾脏中观察到Th 2相关CD 4共受体转录物的产生增加。维生素A单独或与灭活的A.这些数据表明，鱼类暴露于维生素A可能不会引起免疫毒性效应的重大风险。
The objective of this study was to examine the immune toxicity of the alkyl polycyclic aromatic hydrocarbon (PAH) retene (7-isopropyl-1-methyl phenanthrene) in rainbow trout. Retene is a common alkyl PAH associated with combustion of terrestrial plants or industrial effluents. Rainbow trout were injected intraperitoneally with a single dose of 0, 1, or 10mg/kg of retene and sampled at 21d. Within each retene treatment, co-injection of formalin-killed Aeromonas salmonicida or a phosphate buffered saline sham was used to stimulate the trout immune system. At the highest retene dose (10mg/kg) there was an increase in total blood leukocyte counts but only in the A. salmonicida-injected group. This result was paralleled by an increase in leukocytes in differential blood cell counts. There was an overall increase in A. salmonicida-specific antibody titre due to the antigen injection and there was also a significant stimulation of antibody production response at the 10mg/kg retene dose. At the tissue level, immunohistochemistry revealed a greater density of B-lymphocytes at the highest retene dose in spleen and head kidney. A number of immune specific transcripts including the Th2 marker CD4, TCR, MHCII TNFα, and the Th1 marker CD8, INFγ were examined by quantitative RT-PCR in spleen and head kidney. There was no influence of the A. salmonicida antigen on the expression of Th1 related genes in either tissue but increased production of Th2 related CD4 co-receptor transcripts was observed in spleen at both retene concentrations. Retene appeared to be mildly immunostimulatory, either on its own, or in combination with an inactivated A. salmonicida immune challenge and these data suggest that exposures of fishes to retene may not pose significant risk of eliciting immunotoxic effects.
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影响因子：
10.4
作者：
Mills LJ;Gutjahr-Gobell RE;Horowitz DB;Denslow ND;Chow MC;Zaroogian GE
通讯作者：
Zaroogian GE
暴露于漂白牛皮纸制浆厂废水的白吸盘（Catostomus commersoni）肝脏乙氧基试卤灵-O-脱乙基酶活性的第二轮实验室间比较
影响因子：
4.1
作者：
M. Heuvel;D. Dixon;K. Munkittrick;J. Stegeman
通讯作者：
J. Stegeman
短鳍鳗（Anguilla Australis）原位暴露于纸浆和造纸废水中的反应
DOI：
--
发表时间：
2006
期刊：
Journal of Toxicology and Environmental Health, Part A
影响因子：
--
作者：
M. R. van den Heuvel;M. Landman;L. Tremblay
通讯作者：
L. Tremblay
DOI：
--
发表时间：
1996
期刊：
影响因子：
--
作者：
T. Wlklund;G. Bylund
通讯作者：
G. Bylund
影响因子：
3.8
作者：
Gao, J;Lauer, FT;Burchiel, SW
通讯作者：
Burchiel, SW