Differential Expression of Long Noncoding RNAs Reveals a Potential Biomarker for Intractable Pemphigus.
Differential Expression of Long Noncoding RNAs Reveals a Potential Biomarker for Intractable Pemphigus.
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长非编码 RNA 的差异表达揭示了顽固性天疱疮的潜在生物标志物
DOI：
10.1155/2021/5594659
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Pan M
中科院分区：
文献类型：
--
作者：
Qu P;Huang Z;Zhu H;Zheng J;Pan M
长链非编码rna （lncRNAs）参与自身免疫性疾病。然而，lncrna在天疱疮中的作用仍然难以捉摸。目的研究天疱疮患者中lncRNA的表达谱，以确定一种循环lncRNA作为天疱疮的新生物标志物。方法采用lncRNA芯片检测外周血单个核细胞（PBMCs） lncRNA的全局表达谱。差异表达的lncrna通过定量逆转录- pcr （qRT-PCR）验证。通过生物信息学分析了差异表达lncrna的功能和生物学过程。结果lncRNA ENST00000585297在天疱疮患者的PBMCs中表达高于hcc和BP患者。受试者工作特征（ROC）曲线下面积为0.846（95%可信区间(CI) = 0.7526 ~ 0.9397）。有趣的是，我们发现在4周内症状无法控制的天疱疮患者中，与其他症状在4周或更短时间内得到控制的患者相比，ENST00000585297的表达上调（P < 0.05）。此外，ENST00000585297在天疱疮患者中的表达与治疗该疾病所需的泼尼松剂量呈正相关（r = 0.4905, P = 0.0094）。基于竞争内源RNA （ceRNA）理论，构建了竞争内源RNA调控网络。进一步验证表明，ENST00000585297的沉默增加了miR-584-3p的表达。结论本研究首次揭示了天疱疮患者中lncRNAs的表达谱。此外，我们的研究确定了ENST00000585297作为难治性天疱疮病程的生物标志物和指标。
Background Long noncoding RNAs (lncRNAs) are involved in autoimmune diseases. However, the role of lncRNAs in pemphigus remains elusive. Objective The study is aimed at investigating the expression profile in pemphigus patients to identify a circulating lncRNA as a novel biomarker for pemphigus. Method A global lncRNA expression profile in peripheral blood mononuclear cells (PBMCs) was measured by lncRNA microarray. Differentially expressed lncRNAs were validated by quantitative reverse transcriptase-PCR (qRT-PCR). The functional and biological processes of the differentially expressed lncRNAs were analyzed by bioinformatics. Results lncRNA ENST00000585297 in the PBMCs of pemphigus patients was highly expressed compared with those of HCs and BP patients. The area under the receiver operating characteristic (ROC) curve was 0.846 (95%confidence interval (CI) = 0.7526 to 0.9397). Intriguingly, we found that the expression of ENST00000585297 was upregulated in pemphigus patients whose symptoms could not be managed within four weeks compared to other patients whose symptoms could be managed in four weeks or less (P < 0.05). In addition, ENST00000585297 expression in pemphigus patients was positively correlated with the dosage of prednisone needed to manage the disorder (r = 0.4905, P = 0.0094). A competing endogenous RNA (ceRNA) regulatory network was constructed based on the ceRNA theory. Further verification demonstrated that silencing of ENST00000585297 increased the expression of miR-584-3p. Conclusions Our study revealed for the first time the expression profile of lncRNAs in pemphigus patients. In addition, our study identified ENST00000585297 as a biomarker and indicator for the intractable course of pemphigus.
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发表时间：
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影响因子：
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影响因子：
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DOI：
10.1038/s41580-020-00315-9
发表时间：
2021-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
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作者：
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通讯作者：
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