Rapid Generation of Barley Mutant Lines With High Nitrogen Uptake Efficiency by Microspore Mutagenesis and Field Screening.
Rapid Generation of Barley Mutant Lines With High Nitrogen Uptake Efficiency by Microspore Mutagenesis and Field Screening.
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DOI：
10.3389/fpls.2018.00450
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发表时间：
2018
影响因子：
5.6
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Gao R;Guo G;Fang C;Huang S;Chen J;Lu R;Huang J;Fan X;Liu C
通过分离小孢子培养进行体外诱变，可获得大量具有突变导入和纯合性稳定的双单倍体品系，可用于直接筛选。本研究以啤酒大麦为材料，获得了356个DH系。用甲烷磺酸乙酯或平阳霉素对华30号小孢子进行体外诱变处理。在高氮(HN)和低氮(LN)条件下对品系进行田间筛选，以有效分蘖数为主要筛选指标。经过连续3年的筛选，获得了5个高产株数突变系(A1-28、A1-84、A1-226、A16-11和A9-29)。在第5年，以氮素吸收效率(NUPE)、氮素利用效率(NUTE)和氮素转运效率(NTE)为基础，对这些品系的氮素积累、利用和转运等与氮素利用效率(NUE)相关的成分进行表征。结果表明，4个突变株系(A1-84、A1-226、A9-29和A16-11)在HN条件下的NUPE显著提高，而A1-84和A9-29突变株系在LN条件下的NUPE显著提高。结果，他们的净收入有了很大提高。在五个突变系中的任何一个中都没有观察到纽特的改善。A1-84和A9-29是氮素吸收的增强基因型，A1-28在灌浆期表现出较好的氮效率。我们的结果表明，通过小孢子诱变和田间筛选可以获得高NUPE突变体，大麦NUE的提高依赖于氮素吸收的增强。
In vitro mutagenesis via isolated microspore culture provides an efficient way to produce numerous double haploid (DH) lines with mutation introduction and homozygosity stabilization, which can be used for screening directly. In this study, 356 DH lines were produced from the malt barley (Hordeum vulgare L.) cultivar Hua-30 via microspore mutagenic treatment with ethyl methane sulfonate or pingyangmycin during in vitro culture. The lines were subjected to field screening under high nitrogen (HN) and low nitrogen (LN) conditions, and the number of productive tillers was used as the main screening index. Five mutant lines (A1-28, A1-84, A1-226, A16-11, and A9-29) with high numbers of productive tillers were obtained over three consecutive years of screening. In the fifth year, components related to N-use efficiency (NUE), including N accumulation, utilization, and translocation, were characterized for these lines based on N uptake efficiency (NUpE), N utilization efficiency (NUtE), and N translocation efficiency (NTE). The results show that the NUpE of four mutant lines (A1-84, A1-226, A9-29, and A16-11) improved significantly under HN, whereas that of two lines (A1-84 and A9-29) improved under LN. As a result, their NUE improved greatly. No improvement in NUtE was observed in any of the five mutant lines. A1-84 and A9-29 were selected as an enhanced genotype in N uptake, and A1-28 showed improved NTE at the grain-filling stage. Our results imply that high-NUpE mutants can be produced through microspore mutagenesis and field screening, and that improvement of NUE in barley depends on enhancement of N uptake.
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影响因子：
3.4
作者：
Soriano, Mercedes;Li, Hui;Boutilier, Kim
通讯作者：
Boutilier, Kim
影响因子：
5.4
作者：
Liu, Chao;Wang, Jilin;Liu, Kede
通讯作者：
Liu, Kede
通过改善中国集约农业系统的氮肥管理来降低环境风险
DOI：
10.1073/pnas.0813417106
发表时间：
2009-03-03
影响因子：
11.1
作者：
Ju, Xiao-Tang;Xing, Guang-Xi;Zhang, Fu-Suo
通讯作者：
Zhang, Fu-Suo
影响因子：
5.8
作者：
BAETHGEN, WE;CHRISTIANSON, CB;LAMOTHE, AG
通讯作者：
LAMOTHE, AG
影响因子：
6.9
作者：
Sylvester-Bradley, Roger;Kindred, Daniel R.
通讯作者：
Kindred, Daniel R.