Histopathologic correlation with diffusion tensor imaging after chronic hypoxia in the immature ferret
Histopathologic correlation with diffusion tensor imaging after chronic hypoxia in the immature ferret
复制标题
未成熟雪貂慢性缺氧后组织病理学与弥散张量成像的相关性
作者：
J. Tao;A. Barnette;J. Griffith;J. Neil;T. Inder
简介：啮齿动物慢性缺氧引起的白质（WM）损伤与人类早产儿相似。我们采用弥散张量成像（DTI）和免疫组织化学方法研究了缺氧对未成熟雪貂在早产和足月脑相关的两个发育时间点的影响。结果：体外显像显示，缺氧10天后(出生后第10天（P10）至第20天缺氧，并在第20天缺氧时死亡（早期缺氧P20），整个WM的表观扩散系数（ADC）下降，相应的星形细胞增生增加，髓鞘形成减少。正常缺氧10天后（P10至P20缺氧，P30缺氧）弥散值恢复正常，但免疫组化显示明显的星形细胞增生和髓鞘形成降低。相比之下，缺氧10 d后，ADC和各向异性在较晚的发育时间点（P20至P30缺氧，P30缺氧死亡）增加，星形细胞增生减少，髓鞘形成更突出。讨论：影像学和组织学改变的模式与缺氧发生的发育时间有关。扩散测量的归一化与基础组织病理学的归一化不一致。方法：将雪貂进行10%低氧处理，分为早期低氧P20组、早期低氧P30组和晚期低氧P30组。
Introduction:Chronic hypoxia in rodents induces white matter (WM) injury similar to that in human preterm infants. We used diffusion tensor imaging (DTI) and immunohistochemistry to study the impact of hypoxia in the immature ferret at two developmental time points relevant to the preterm and term brain.Results:On ex vivo imaging, the apparent diffusion coefficient (ADC) was decreased throughout the WM after 10 days of hypoxia (hypoxia from postnatal day 10 (P10) to P20 and killed at P20 (early hypoxia P20)), corresponding to increased astrocytosis and decreased myelination. Diffusion values normalized after 10 days of normoxia (hypoxia from P10 to P20 and killed at P30 (early hypoxia P30)), but immunohistochemistry revealed significant astrocytosis and hypomyelination. In contrast, ADC and anisotropy were increased after 10 days of hypoxia at a later developmental time point (hypoxia from P20 to P30 and killed at P30 (late hypoxia P30)), with less astrocytosis and more prominent myelination.Discussion:The patterns of alteration in imaging and histology varied in relation to the developmental time at which hypoxia occurred. Normalization of diffusion measures did not correspond to the normalization of underlying histopathology.Methods:Ferrets were subjected to 10% hypoxia and divided into three groups: early hypoxia P20, early hypoxia P30, and late hypoxia P30.
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影响因子：
3.7
作者：
Chahboune, Halima;Ment, Laura R.;Schwartz, Michael L.
通讯作者：
Schwartz, Michael L.
慢性新生儿缺氧导致大鼠大脑皮层的体积和细胞数量长期减少。
影响因子：
3.4
作者：
Schwartz,MichaelL;Vaccarino,Flora;Chacon,Monica;Yan,WeiLi;Ment,LauraR;Stewart,WilliamB
通讯作者：
Stewart,WilliamB
影响因子：
3.7
作者：
McKinstry, RC;Mathur, A;Neil, JJ
通讯作者：
Neil, JJ
影响因子：
14.5
作者：
Dubois, J.;Benders, M.;Hueppi, P. S.
通讯作者：
Hueppi, P. S.