Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes.
Inflammation-induced endothelial cell-derived extracellular vesicles modulate the cellular status of pericytes.
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DOI：
10.1038/srep08505
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发表时间：
2015-02-17
影响因子：
4.6
通讯作者：
Sasahara M
中科院分区：
文献类型：
--
作者：
Yamamoto S;Niida S;Azuma E;Yanagibashi T;Muramatsu M;Huang TT;Sagara H;Higaki S;Ikutani M;Nagai Y;Takatsu K;Miyazaki K;Hamashima T;Mori H;Matsuda N;Ishii Y;Sasahara M
新出现的证据表明，细胞外小泡(EV)通过将包裹的物质，如microRNAs(MiRNAs)输出到靶细胞来介导细胞间的通讯。血管内皮细胞衍生的血管内皮生长因子(E-EVS)在不同的病理条件下在循环血液中表达上调，但其特征和作用尚不清楚。体外研究确定了炎症诱导的E-EV在血管内皮细胞和周细胞/VSMC之间的细胞间通讯中的作用。在炎性细胞因子和内毒素的刺激下，血管内皮细胞立即释放脱落型E-EV，流式细胞仪检测结果显示这种诱导作用具有剂量依赖性。MiRNA阵列分析显示，在炎症诱导的E-EVS中，一组miRNAs特异性增加。将加入脑血管周细胞培养液中的E-EV掺入细胞内。添加E-EV的细胞显示出高度诱导的VEGF-B的mRNA和蛋白表达，这被认为是炎症刺激后E-EV内增加的miRNA的下游靶点。结果提示，E-EVS介导炎症诱导的内皮细胞-周细胞/vSMC通讯，包裹在E-EVS中的miRNAs可能在调节靶细胞功能中发挥作用。E-EVS可能成为治疗炎症性疾病的新靶点。
Emerging lines of evidence have shown that extracellular vesicles (EVs) mediate cell-to-cell communication by exporting encapsulated materials, such as microRNAs (miRNAs), to target cells. Endothelial cell-derived EVs (E-EVs) are upregulated in circulating blood in different pathological conditions; however, the characteristics and the role of these E-EVs are not yet well understood. In vitro studies were conducted to determine the role of inflammation-induced E-EVs in the cell-to-cell communication between vascular endothelial cells and pericytes/vSMCs. Stimulation with inflammatory cytokines and endotoxin immediately induced release of shedding type E-EVs from the vascular endothelial cells, and flow cytometry showed that the induction was dose dependent. MiRNA array analyses revealed that group of miRNAs were specifically increased in the inflammation-induced E-EVs. E-EVs added to the culture media of cerebrovascular pericytes were incorporated into the cells. The E-EV-supplemented cells showed highly induced mRNA and protein expression of VEGF-B, which was assumed to be a downstream target of the miRNA that was increased within the E-EVs after inflammatory stimulation. The results suggest that E-EVs mediate inflammation-induced endothelial cell-pericyte/vSMC communication, and the miRNAs encapsulated within the E-EVs may play a role in regulating target cell function. E-EVs may be new therapeutic targets for the treatment of inflammatory diseases.
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影响因子：
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作者：
Fujita Y;Kuwano K;Ochiya T;Takeshita F
通讯作者：
Takeshita F
DOI：
10.1073/pnas.0911661107
发表时间：
2010-01-12
影响因子：
11.1
作者：
Cao, Renhai;Xue, Yuan;Cao, Yihai
通讯作者：
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影响因子：
3.7
作者：
Hassan F;Nuovo GJ;Crawford M;Boyaka PN;Kirkby S;Nana-Sinkam SP;Cormet-Boyaka E
通讯作者：
Cormet-Boyaka E
DOI：
10.1161/atvbaha.110.218123
发表时间：
2011-01-01
影响因子：
8.7
作者：
Dignat-George, Francoise;Boulanger, Chantal M.
通讯作者：
Boulanger, Chantal M.
DOI：
10.1073/pnas.1017667108
发表时间：
2011-03-22
影响因子：
11.1
作者：
Antonyak, Marc A.;Li, Bo;Cerione, Richard A.
通讯作者：
Cerione, Richard A.