Diversity of culturable heterotrophic bacteria from the Mariana Trench and their ability to degrade macromolecules
Diversity of culturable heterotrophic bacteria from the Mariana Trench and their ability to degrade macromolecules
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马里亚纳海沟可培养异养细菌的多样性及其降解大分子的能力
DOI：
10.1007/s42995-020-00027-1
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发表时间：
2020-02
影响因子：
5.7
通讯作者：
Xiao-Hua Zhang
中科院分区：
文献类型：
--
作者：
Xiuxiu Zhao;Jiwen Liu;Shun Zhou;Yanfen Zheng;Yanhong Wu;Kazuhiro Kogure;Xiao-Hua Zhang
The Mariana Trench is the deepest location on earth and harbors unique microbial communities as evidenced by 16S rRNA gene amplicon and metagenomic sequencing. Obtaining culturable microorganisms from the Mariana Trench will contribute to a further understanding of hadal biogeochemical processes and act as a unique microbial reservoir with potential applications. Here, 825 bacterial strains, identified by 16S rRNA gene sequencing, were isolated from 12 water depths (0–10,400 m) of the Mariana Trench with 2216E and R2A media at 4 °C or 28 °C on four cruises during 2015–2017. These bacteria belong to four phyla, nine classes, 27 orders, 45 families and 108 genera.Alphaproteobacteria,Gammaproteobacteria,Actinobacteria_c,BacilliandFlavobacteriiawere the most abundant classes, accounting for 37.9%, 33.0%, 11.8%, 8.6% and 8.0% of the total bacterial isolates, respectively. 2216E and R2A media were found to have a better selectivity toBacilliandFlavobacteriia, respectively. Fifty strains were potential novel bacterial species with a 16S rRNA gene similarity < 98.65%, and a higher percentage of novel strains were obtained from R2A than 2216E medium. Additionally, 301 (150 species) out of 354 strains (178 species) selected from each depth could degrade at least one of the ten kinds of macromolecules tested. These results indicate that there is a high diversity of culturable bacteria in the Mariana Trench and they can produce a variety of extracellular enzymes. Our study provides a valuable resource of microorganisms for investigating their biogeochemical roles in the Mariana Trench and for industrial applications.
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马里亚纳海沟内部异养细菌几乎均匀的分布模式
DOI：
10.1016/j.dsr.2018.10.002
发表时间：
2018-12
期刊：
Deep-Sea Research Part I
影响因子：
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作者：
Tian Jiwei;Fan Lu;Liu H.D.;Liu J.;Li Y.;Qin Q.;Gong Z.;Chen H.T.;Sun Z.B.;Zou L;Wang X.;Xu H.;Bartlett D.;Wang M.;Zhang Y.Z.;Zhang X.H.;Zhang C.L
通讯作者：
Zhang C.L
DOI：
10.1099/ijsem.0.001671
发表时间：
2017-04
影响因子：
2.8
作者：
M. Kusube;Than S. Kyaw;Kumiko Tanikawa;R. Chastain;Kevin Hardy;James Cameron;D. Bartlett
通讯作者：
M. Kusube;Than S. Kyaw;Kumiko Tanikawa;R. Chastain;Kevin Hardy;James Cameron;D. Bartlett
DOI：
10.1099/ijs.0.008193-0
发表时间：
2009-12
影响因子：
2.8
作者：
Qiliang Lai;Jun Yuan;Z. Shao
通讯作者：
Qiliang Lai;Jun Yuan;Z. Shao
影响因子：
5.2
作者：
Fujioka, K;Okino, K;Ohara, Y
通讯作者：
Ohara, Y
影响因子：
2.8
作者：
KOGURE, K;SIMIDU, U;TAGA, N
通讯作者：
TAGA, N