Bioactive and bioresorbable cellular cubic-composite scaffolds for use in bone reconstruction
Bioactive and bioresorbable cellular cubic-composite scaffolds for use in bone reconstruction
复制标题
用于骨重建的生物活性和生物可吸收细胞立方复合支架
DOI：
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发表时间：
2006
影响因子：
3.9
通讯作者：
Takashi Nakamura
中科院分区：
文献类型：
--
作者：
Y. Shikinami;Kenshi Okazaki;Makoto Saito;M. Okuno;Shin Hasegawa;J. Tamura;S. Fujibayashi;Takashi Nakamura
我们使用一种新的复合纤维沉淀法在聚-d/l-丙交酯基质中制备含有磷酸钙（CaP）颗粒（未烧结和未煅烧的羟基磷灰石（u-HA）、α-磷酸三钙、β-磷酸三钙、磷酸四钙、磷酸二钙二水合物、磷酸二钙无水物或磷酸八钙）的生物活性和生物可吸收的细胞立方体复合材料。CaP颗粒在复合材料中占据大于或等于70wt%（大于或等于50vol%）的分数。 蜂窝状立方复合材料的孔隙率大于或等于70%，并且互连孔占这些值的大于或等于70%。随着时间的推移，在体外的细胞的几何形状和复合材料的物理性能的变化进行了评价。采用碱性磷酸酶法检测成骨细胞的分化，而Alcohol Blue法检测成骨细胞的增殖。在30重量%聚-d/l-丙交酯基质中含有70重量% u-HA颗粒的细胞立方体C-u-HA 70在所测试的材料中显示出最大的三维细胞亲和力。 该复合材料具有与松质骨相似的抗压强度和细胞几何形状，可在术中进行改良（通过修剪或加热），并能够形成皮质-松质骨样杂交体。C-u-HA 70的骨诱导性，独立于生物生长因子，通过植入到比格犬的背部肌肉中来证实。我们的研究结果表明，C-u-HA 70具有作为细胞支架或临时硬组织替代物用于骨重建的临床应用的潜力。
We used a novel composite fibre-precipitation method to create bioactive and bioresorbable cellular cubic composites containing calcium phosphate (CaP) particles (unsintered and uncalcined hydroxyapatite (u-HA), α-tricalcium phosphate, β-tricalcium phosphate, tetracalcium phosphate, dicalcium phosphate dihydrate, dicalcium phosphate anhydrate or octacalcium phosphate) in a poly-d/l-lactide matrix. The CaP particles occupied greater than or equal to 70 wt% (greater than or equal to 50 vol%) fractions within the composites. The porosities of the cellular cubic composites were greater than or equal to 70% and interconnective pores accounted for greater than or equal to 70% of these values. In vitro changes in the cellular geometries and physical properties of the composites were evaluated over time. The Alamar Blue assay was used to measure osteoblast proliferation, while the alkaline phosphatase assay was used to measure osteoblast differentiation. Cellular cubic C-u-HA70, which contained 70 wt% u-HA particles in a 30 wt% poly-d/l-lactide matrix, showed the greatest three-dimensional cell affinity among the materials tested. This composite had similar compressive strength and cellular geometry to cancellous bone, could be modified intraoperatively (by trimming or heating) and was able to form cortico-cancellous bone-like hybrids. The osteoinductivity of C-u-HA70, independent of biological growth factors, was confirmed by implantation into the back muscles of beagles. Our results demonstrated that C-u-HA70 has the potential as a cell scaffold or temporary hard-tissue substitute for clinical use in bone reconstruction.
影响因子：
14
作者：
Burdick, JA;Frankel, D;Anseth, KS
通讯作者：
Anseth, KS
影响因子：
1.3
作者：
LOTZ, JC;GERHART, TN;HAYES, WC
通讯作者：
HAYES, WC