Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.).
Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.).
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大白菜根肿病抗性位点CRA8.1精细定位及候选基因分析
DOI：
10.3389/fpls.2022.898108
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
根肿病是由芸苔根肿菌（Plasmodiophora brassicae）引起的一种重要病害，严重威胁着世界范围内的芸苔科作物生产。近年来，根肿病在我国十字花科作物主产区大面积发生并迅速蔓延。本研究从我国目前主要抗根肿病的甘蓝型油菜品种华双5 R（H5 R）和华油杂62 R中筛选出抗根肿病能力不同的甘蓝材料定温（DW）。我们使用了新一代的基于测序的大规模分离分析方法，结合遗传作图，以确定根肿病抗性（CR）基因从F1群体之间的DW（CR）和Haponix（根肿病敏感）之间的交叉产生。将DW的CR位点（命名为CRA8.1）定位在标记A08-4346和A08-4853之间的区域，该区域含有两个不同的位点CRA8.1a和CRA8.1b。CRA8.1b基因座位于A08染色体上A08-4624和A08-4853之间，约395 kb，与PbZj和PbXm分离物的抗性有关。然而，与对应于标记A08-4346和A08-4624之间的765-kb区域的CRA8.1a一起，它可以赋予对PbXm+的抗性。最后，通过抗病材料和感病材料的表达分析，确定了两个编码TIR-NBS-LRR蛋白的基因（BraA 08 g 039211 E和BraA 08 g 039212 E）和一个编码RLP蛋白的基因（BraA 08 g 039193 E）最有可能是DW特异抗性的CR候选基因。
Clubroot is caused by Plasmodiophora brassicae, which threatens Brassicaceae crop production worldwide. In recent years, there has been an outbreak and rapid spread of clubroot in many major cruciferous crop-producing areas of China. In this study, we identified a cabbage material DingWen (DW) with different resistant capabilities from Huashuang5R (H5R) and Huayouza62R of Brassica napus, which are currently used as the main resistant cultivars for clubroot management in China. We used a next-generation sequencing-based bulked segregant analysis approach, combined with genetic mapping to identify clubroot-resistant (CR) genes from F1 population generated from a cross between the DW (CR) and HZSX (clubroot susceptible). The CR locus of DW (named CRA8.1) was mapped to a region between markers A08-4346 and A08-4853, which contains two different loci CRA8.1a and CRA8.1b after fine mapping. The CRA8.1b loci contain a fragment of 395 kb between markers A08-4624 and A08-4853 on A08 chromosome, and it is responsible for the resistance to PbZj and PbXm isolates. However, together with CRA8.1a, corresponding to a 765-kb region between markers A08-4346 and A08-4624, then it can confer resistance to PbXm+. Finally, through expression analysis between resistant and susceptible materials, two genes encoding TIR-NBS-LRR proteins (BraA08g039211E and BraA08g039212E) and one gene encoding an RLP protein (BraA08g039193E) were identified to be the most likely CR candidates for the peculiar resistance in DW.
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影响因子：
3.7
作者：
Hatakeyama K;Suwabe K;Tomita RN;Kato T;Nunome T;Fukuoka H;Matsumoto S
通讯作者：
Matsumoto S
影响因子：
5.6
作者：
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通讯作者：
Strelkov, Stephen E.
影响因子：
5.6
作者：
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通讯作者：
Yu, Fengqun
影响因子：
2.7
作者：
Fredua-Agyeman, R.;Hwang, S. -F.;Feindel, D.
通讯作者：
Feindel, D.
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影响因子：
5.6
作者：
Li Q;Shah N;Zhou X;Wang H;Yu W;Luo J;Liu Y;Li G;Liu C;Zhang C;Chen P
通讯作者：
Chen P