Identification of Micro Ribonucleic Acids and Their Targets in Response to Plasmodiophora brassicae Infection in Brassica napus.
Identification of Micro Ribonucleic Acids and Their Targets in Response to Plasmodiophora brassicae Infection in Brassica napus.
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甘蓝型油菜中响应甘蓝根霉感染的微核糖核酸及其靶标的鉴定
DOI：
10.3389/fpls.2021.734419
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发表时间：
2021
影响因子：
5.6
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Li Q;Shah N;Zhou X;Wang H;Yu W;Luo J;Liu Y;Li G;Liu C;Zhang C;Chen P
根肿病（Clubroot disease）是由土传病原菌芸苔根肿菌（Plasmodiophora brassicae War）引起的世界上最古老、最具破坏性的芸苔属和十字花科作物病害之一。植物microRNA [microribonucleicacids（miRNAs）]在许多发育过程中起着重要的调控作用。虽然植物miRNAs在植物-微生物相互作用中的作用已被广泛研究，但关于miRNAs在响应芸苔疫霉的特定功能的报道很少。本研究以甘蓝型油菜抗根肿病近等基因系409 R和感根肿病近等基因系409 S为材料，研究了甘蓝型油菜根肿病侵染过程中miRNAs及其靶基因的作用。在接种或不接种芸苔疫霉的409 R和409 S的根样品上进行小RNA测序（sRNA-seq）和降解组-seq。sRNA-seq共鉴定出48个保守的miRNAs和72个新的miRNAs，其中18个在409 R的根部差异表达，而只有1个miRNAs在409 S的根部差异表达。degradome-seq分析鉴定了938种miRNA靶转录物，它们是参与多种生物过程的转录因子、酶和蛋白质，并且在植物激素信号转导途径中最显著富集。在409 R和409 S之间，我们发现了八种不同的降解途径，如与脂肪酸相关的降解途径。通过结合已发表的转录组数据，我们在409 R中鉴定了总共6个拮抗性miRNA-靶标对，它们对芸苔疫霉感染有反应，并参与与根发育、过敏性细胞死亡和叶绿体代谢合成相关的途径。我们的结果表明，芸苔疫霉感染导致miRNA库和靶转录本发生巨大变化。更有趣的是，这些变化在409 R和409 S之间是不同的。阐明miRNAs与靶标之间的相互作用可能为病原菌对芸苔疫霉抗性的可能机制提供新的线索。
Clubroot disease, which is caused by the soil-borne pathogen Plasmodiophora brassicae War (P. brassicae), is one of the oldest and most destructive diseases of Brassica and cruciferous crops in the world. Plant microRNAs [micro ribonucleic acids (miRNAs)] play important regulatory roles in several developmental processes. Although the role of plant miRNAs in plant-microbe interaction has been extensively studied, there are only few reports on the specific functions of miRNAs in response to P. brassicae. This study investigated the roles of miRNAs and their targets during P. brassicae infection in a pair of Brassica napus near-isogenic lines (NILs), namely clubroot-resistant line 409R and clubroot-susceptible line 409S. Small RNA sequencing (sRNA-seq) and degradome-seq were performed on root samples of 409R and 409S with or without P. brassicae inoculation. sRNA-seq identified a total of 48 conserved and 72 novel miRNAs, among which 18 had a significant differential expression in the root of 409R, while only one miRNA was differentially expressed in the root of 409S after P. brassicae inoculation. The degradome-seq analysis identified 938 miRNA target transcripts, which are transcription factors, enzymes, and proteins involved in multiple biological processes and most significantly enriched in the plant hormone signal transduction pathway. Between 409R and 409S, we found eight different degradation pathways in response to P. brassicae infection, such as those related to fatty acids. By combining published transcriptome data, we identified a total of six antagonistic miRNA-target pairs in 409R that are responsive to P. brassicae infection and involved in pathways associated with root development, hypersensitive cell death, and chloroplast metabolic synthesis. Our results reveal that P. brassicae infection leads to great changes in miRNA pool and target transcripts. More interestingly, these changes are different between 409R and 409S. Clarification of the crosstalk between miRNAs and their targets may shed new light on the possible mechanisms underlying the pathogen resistance against P. brassicae.
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影响因子：
3.7
作者：
Hatakeyama K;Suwabe K;Tomita RN;Kato T;Nunome T;Fukuoka H;Matsumoto S
通讯作者：
Matsumoto S
影响因子：
3.3
作者：
de Vries S;Kloesges T;Rose LE
通讯作者：
Rose LE
DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
拮抗受体的表观遗传调控赋予稻瘟病抗性和产量平衡。
影响因子：
56.9
作者：
Deng, Yiwen;Zhai, Keran;He, Zuhua
通讯作者：
He, Zuhua
影响因子：
3.7
作者：
Chen J;Jing J;Zhan Z;Zhang T;Zhang C;Piao Z
通讯作者：
Piao Z