Evaluation of the bioactivity about anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold for pelvic reconstruction
Evaluation of the bioactivity about anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold for pelvic reconstruction
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抗sca-1/碱性成纤维细胞生长因子-膀胱基质支架用于盆腔重建的生物活性评价
DOI：
10.1177/0885328218811390
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发表时间：
2018-11
影响因子：
2.9
通讯作者：
Huicheng Xu
中科院分区：
文献类型：
--
作者：
Jiankui Li;Xi Chen;Kaijian Ling;Zhiqing Liang;Huicheng Xu
摘要：引言和假设：盆腔支持结构损伤是盆腔器官脱垂的主要原因。目前，聚丙烯基填充材料已被建议作为治疗盆腔器官脱垂的常用方法。然而，它不能在功能上恢复骨盆支撑结构。除了长期效果较差外，膀胱基质是最适合盆底修复的生物支架材料。本研究假设将抗SCA-1单克隆抗体和碱性成纤维细胞生长因子交联于膀胱基质，构建双因子生物支架，用于盆腔重建。研究方法：通过双特异性交联剂，磺基琥珀酰亚胺基4-[N-马来酰亚胺甲基]环己烷-1-羧酸酯（sulfo-smcc）将抗sca-1和碱性成纤维细胞生长因子固定到膀胱基质上。然后用扫描电镜和酶标仪检测抗sca-1/碱性成纤维细胞生长因子-膀胱基质支架是否构建成功。在此基础上，在体外和体内测定了富集SCA-1阳性细胞的能力。此外，我们评估的分化能力和生物相容性的支架。最后，采用western blotting检测fibulin-5蛋白的表达水平结果：扫描电镜和酶标仪显示双因子生物支架构建成功。该支架在体内外均能显著富集大量SCA-1阳性细胞，明显促进细胞分化和功能组织分化，具有良好的生物相容性。Western blotting结果显示，支架材料能促进fibulin-5蛋白的表达.结论：抗sca-1/碱性成纤维细胞生长因子膀胱基质支架具有biological.properties，是一种理想的骨盆重建支架材料。
Abstract.Introduction and hypothesis: Pelvic support structure injury is the major cause of pelvic organ prolapse. At present,.polypropylene-based filler material has been suggested as a common method to treat pelvic organ prolapse. However, it.cannot functionally rehabilitate the pelvic support structure. In addition to its poor long-term efficiency, the urinary.bladder matrix was the most suitable biological scaffold material for pelvic floor repair. Here, we hypothesize that antisca-1 monoclonal antibody and basic fibroblast growth factor were cross-linked to urinary bladder matrix to construct a.two-factor bioscaffold for pelvic reconstruction..Methods: Through a bispecific cross-linking reagent, sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-.carboxylate (sulfo-smcc) immobilized anti-sca-1 and basic fibroblast growth factor to urinary bladder matrix. Then.scanning electron microscope and plate reader were used to detect whether the anti-sca-1/basic fibroblast growth.factor-urinary bladder matrix scaffold was built successfully. After that, the capacity of enriching sca-1 positive cells was.measured both in vitro and in vivo. In addition, we evaluated the differentiation capacity and biocompatibility of the.scaffold. Finally, western blotting was used to detect the level of fibulin-5 protein..Results: The scanning electron microscope and plate reader revealed that the double-factor biological scaffold was built.successfully. The scaffold could significantly enrich a large number of sca-1 positive cells both in vitro and in vivo, and.obviously accelerate cells and differentiate functional tissue with good biocompatibility. Moreover, the western blotting.showed that the scaffold could improve the expression of fibulin-5 protein..Conclusion: The anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold revealed good biological.properties and might serve as an ideal scaffold for pelvic reconstruction.
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