Collagen scaffolds modified with collagen-binding bFGF promotes the neural regeneration in a rat hemisected spinal cord injury model
Collagen scaffolds modified with collagen-binding bFGF promotes the neural regeneration in a rat hemisected spinal cord injury model
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用胶原结合 bFGF 修饰的胶原支架促进大鼠半切脊髓损伤模型的神经再生
DOI：
10.1007/s11427-014-4612-7
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
陈亮
中科院分区：
文献类型：
--
作者：
陈亮
神经导管是脊髓损伤(SCI)的治疗策略之一。最近的研究表明，生物材料可以引导损伤部位的轴突生长，促进轴突再生。然而，支架本身难以满足脊髓损伤功能恢复的需要。碱性成纤维细胞生长因子可显著促进器官损伤后的功能恢复。本实验采用大鼠T9脊髓半横断损伤模型，评价胶原结合碱性成纤维细胞生长因子(CBD-bFGF)修饰的胶原支架(CS)植入后的修复能力。结果表明，CS联合CBD-bFGF治疗可提高脊髓损伤后的存活率。通过21分Basso-Beattie-Bresnahan(BBB)评分和足迹分析，CS/CBD-bFGF组较单纯CS植入组和未治疗对照组在运动方面有更显著的改善。苏木精-伊红(H&E)染色和神经丝(NF)、胶质纤维酸性蛋白(GFAP)免疫组织化学染色显示，纤维被引导通过种植体生长。这些结果表明，应用CBD-bFGF修饰CS可以促进脊髓再生和功能恢复。
Nerve conduit is one of strategies for spine cord injury (SCI) treatment. Recently, studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site. However, the scaffold by itself was difficult to meet the need of SCI functional recovery. The basic fibroblast growth factor (bFGF) administration significantly promotes functional recovery after organ injuries. Here, using a rat model of T9 hemisected SCI, we aimed at assessing the repair capacity of implantation of collagen scaffold (CS) modified by collagen binding bFGF (CBD-bFGF). The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI. The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group, when evaluated by the 21-point Basso-Beattie-Bresnahan (BBB) score and footprint analysis. Both hematoxylin and eosin (H&E) and immunohistochemical staining of neurofilament (NF) and glial fibrillary acidic protein (GFAP) demonstrated that fibers were guided to grow through the implants. These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery.
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影响因子：
14
作者：
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