The role of pH fronts in tissue electroporation based treatments.
The role of pH fronts in tissue electroporation based treatments.
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DOI：
10.1371/journal.pone.0080167
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Marshall G
中科院分区：
文献类型：
--
作者：
Maglietti F;Michinski S;Olaiz N;Castro M;Suárez C;Marshall G
基于电穿孔（EP）的处理，由于脉冲电场的应用，在细胞膜上诱导形成孔。我们提出的实验证据表明，在基于组织EP的治疗过程中，两个电极出现的pH前沿存在，对于许多研究中发现的条件，这些前沿是直接和实质性的。通过消失时间（ET）间接测量pH前沿，该时间定义为组织缓冲液中和它们所需的时间。通过pH指标在一系列时间间隔成像来测量ET，使用四聚类硬模糊c均值算法对每帧pH指标对应的像素进行分割。ET计算为像素数为初始帧像素数的10%的时间。在基于电位的治疗中，如可逆电穿孔（ECT）和不可逆电穿孔（IRE），由于电脉冲特性和组织中存在的生物缓冲，ET非常短（尽管足以造成轻微伤害），而在基因电转移（GET）中，ET要长得多，足以使质粒变性并产生细胞损伤。当任何一个电脉冲参数增加一倍或三倍时，ET增长，值得注意的是，当GET中的任何一个脉冲参数减半时，ET显著下降。由于质粒损伤和细胞损失的大幅减少，降低pH前沿对GET的治疗效率有相关的影响。
Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many studies, and that these fronts are immediate and substantial. pH fronts are indirectly measured through the evanescence time (ET), defined as the time required for the tissue buffer to neutralize them. The ET was measured through a pH indicator imaged at a series of time intervals using a four-cluster hard fuzzy-c-means algorithm to segment pixels corresponding to the pH indicator at every frame. The ET was calculated as the time during which the number of pixels was 10% of those in the initial frame. While in EP-based treatments such as reversible (ECT) and irreversible electroporation (IRE) the ET is very short (though enough to cause minor injuries) due to electric pulse characteristics and biological buffers present in the tissue, in gene electrotransfer (GET), ET is much longer, enough to denaturate plasmids and produce cell damage. When any of the electric pulse parameters is doubled or tripled the ET grows and, remarkably, when any of the pulse parameters in GET is halved, the ET drops significantly. Reducing pH fronts has relevant implications for GET treatment efficiency, due to a substantial reduction of plasmid damage and cell loss.
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影响因子：
3.9
作者：
Arena CB;Sano MB;Rossmeisl JH Jr;Caldwell JL;Garcia PA;Rylander MN;Davalos RV
通讯作者：
Davalos RV
影响因子：
3.4
作者：
CROWLEY, JM
通讯作者：
CROWLEY, JM
影响因子：
3.5
作者：
Bloquel, C;Fabre, E;Scherman, D
通讯作者：
Scherman, D
影响因子：
5
作者：
Colombo, L.;Gonzalez, G.;Turjanski, P.
通讯作者：
Turjanski, P.
影响因子：
5.1
作者：
André, F;Mir, LM
通讯作者：
Mir, LM