Mass spectrometry and next-generation sequencing reveal an abundant and rapidly evolving abalone sperm protein.
Mass spectrometry and next-generation sequencing reveal an abundant and rapidly evolving abalone sperm protein.
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DOI：
10.1002/mrd.22182
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发表时间：
2013-06
影响因子：
2.5
通讯作者：
Swanson, Willie J.
中科院分区：
文献类型：
--
作者：
Palmer, Melody R.;McDowall, Margo H.;Stewart, Lia;Ouaddi, Aleena;MacCoss, Michael J.;Swanson, Willie J.
鲍鱼是一种广播产卵的海洋软体动物，是受精过程中分子相互作用和正选择的重要模型，但之前的焦点仅集中在两种精子蛋白：溶素和 sp18。我们利用基因组和蛋白质组技术，通过表征红鲍的睾丸转录组和精子蛋白质组，为该模型带来了新的见解。一对同源的睾丸特异性蛋白质含有分泌信号，并且体积小、含量丰富且与顶体相关。比较分析表明，同源物在物种之间存在极大差异，并显示出积极选择的有力证据。这些蛋白质的顶体定位和快速进化表明它们在受精中发挥重要作用，并可能参与鲍鱼精卵相互作用的物种特异性。我们对鲍鱼受精的基因组和蛋白质组学特征进行了鉴定，结果发现了一些有趣的新型肽，这些肽在这个重要的模型系统中二十年来一直未能被检测到。
Abalone, a broadcast spawning marine mollusk, is an important model for molecular interactions and positive selection in fertilization, but the focus has previously been on only two sperm proteins, lysin and sp18.We used genomic and proteomic techniques to bring new insights to this model by characterizing the testis transcriptome and sperm proteome of the Red abalone Haliotis rufescens. One pair of homologous, testis-specific proteins contains a secretion signal and is small, abundant, and associated with the acrosome. Comparative analysis revealed that homologs are extremely divergent between species, and show strong evidence for positive selection. The acrosomal localization and rapid evolution of these proteins indicates that they play an important role in fertilization, and could be involved in the species-specificity of sperm-egg interactions in abalone. Our genomic and proteomic characterization of abalone fertilization resulted in the identification of interesting, novel peptides that have eluded detection in this important model system for 20 years.
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DOI：
10.1073/pnas.0603125103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Aagaard, Jan E.;Yi, Xianhua;Swanson, Willie J.
通讯作者：
Swanson, Willie J.
DOI：
10.1016/s0022-5320(80)90133-1
发表时间：
1980-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
LEWIS, CA;LEIGHTON, DL;VACQUIER, VD
通讯作者：
VACQUIER, VD
影响因子：
56.9
作者：
MORSE, DE;DUNCAN, H;MORSE, A
通讯作者：
MORSE, A
影响因子：
4.9
作者：
Pujolar, J. M.;Pogson, G. H.
通讯作者：
Pogson, G. H.
影响因子：
3.9
作者：
Schully, Sheri Dixon;Hellberg, Michael E.
通讯作者：
Hellberg, Michael E.