Staphylococcus aureus temperate bacteriophage: carriage and horizontal gene transfer is lineage associated.
Staphylococcus aureus temperate bacteriophage: carriage and horizontal gene transfer is lineage associated.
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DOI：
10.3389/fcimb.2012.00006
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发表时间：
2012
影响因子：
5.7
通讯作者：
Lindsay JA
中科院分区：
文献类型：
--
作者：
McCarthy AJ;Witney AA;Lindsay JA
金黄色葡萄球菌是人类和动物感染的主要原因。噬菌体是一类携带毒力基因并水平传播的移动遗传元件（MGE），包括潘顿-瓦伦丁杀白细胞素（PVL）、与人类特异性相关的免疫逃避簇（IEC）和与食物中毒相关的主要毒素A肠毒素。金黄色葡萄球菌将群体分离成主要克隆复合体 (CC) 谱系，这些谱系由于拥有不同的限制修饰 (RM) 系统而在很大程度上独立进化。我们的目的是通过使用（i）生物信息学方法分析前 79 个已测序的金黄色葡萄球菌分离株的噬菌体基因组，以及（ii）金黄色葡萄球菌微阵列来分析来自更广泛谱系的金黄色葡萄球菌分离株中噬菌体和毒力基因的分布，从而更好地了解噬菌体毒力和抗性基因的水平和垂直传播动态。八个噬菌体家族的分布变化很大，但谱系相关。然而，有证据表明，频繁的获取和丢失而不仅仅是垂直传播。大多数噬菌体基因都是可有可无的，并且可以看到广泛的嵌合体。令人惊讶的是，毒力基因与特定噬菌体家族密切相关。该数据表明，金黄色葡萄球菌噬菌体进化迅速，噬菌体编码的毒力基因的水平基因转移（HGT）受到噬菌体家族和宿主细菌谱系的限制，延迟了完全耐药和强毒菌株的进化。
Staphylococcus aureus is a major cause of human and animal infections. Bacteriophage are a class of mobile genetic element (MGE) that carry virulence genes and disseminate them horizontally, including Panton–Valentine leukocidin (PVL), the immune evasion cluster (IEC) associated with human specificity, and enterotoxin A the major toxin associated with food poisoning. S. aureus isolates group into major clonal complex (CC) lineages that largely evolve independently due to possession of different restriction–modification (RM) systems. We aimed to better understand the horizontal and vertical transmission dynamics of virulence and resistance genes by bacteriophage by using (i) bioinformatic approaches to analyze bacteriophage genomes from the first 79 sequenced S. aureus isolates and (ii) S. aureus microarrays to analyze the distribution of bacteriophage and virulence genes in S. aureus isolates from a broader range of lineages. The distribution of eight bacteriophage families was highly variable but lineage associated. Nevertheless, there was evidence of frequent acquisition and loss and not just vertical transmission. Most bacteriophage genes were dispensable, and extensive mosaicism was seen. Surprisingly, virulence genes were tightly associated with specific phage families. This data suggests S. aureus bacteriophage evolve rapidly, and the horizontal gene transfer (HGT) of virulence genes encoded by bacteriophage is restricted by bacteriophage family and the lineage of the host bacterium, delaying the evolution of fully resistant and virulent strains.
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影响因子：
3.2
作者：
Lindsay, JA;Moore, CE;Hinds, J
通讯作者：
Hinds, J
影响因子：
56.9
作者：
BETLEY, MJ;MEKALANOS, JJ
通讯作者：
MEKALANOS, JJ
影响因子：
3.6
作者：
Goerke, Christiane;Wirtz, Christiane;Wolz, Christiane
通讯作者：
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影响因子：
6.4
作者：
O'Hara, F. Patrick;Guex, Nicolas;Amrine-Madsen, Heather
通讯作者：
Amrine-Madsen, Heather
影响因子：
3.5
作者：
Kaneko, J;Kimura, T;Kamio, Y
通讯作者：
Kamio, Y