Simultaneous determination of four phenolic acids in traditional Chinese medicine by capillary electrophoresis-chemiluminescence.
Simultaneous determination of four phenolic acids in traditional Chinese medicine by capillary electrophoresis-chemiluminescence.
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毛细管电泳-化学发光法同时测定中药中四种酚酸类物质
DOI：
10.1039/d1ra06608k
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发表时间：
2021-10-18
期刊：
影响因子：
3.9
通讯作者：
Xu, Xiangdong
中科院分区：
文献类型：
--
作者：
Yang, Shuopeng;Han, Yanzhen;Wang, Kairui;Wang, Yu;Li, Liping;Li, Nan;Xu, Xiangdong
绿原酸、阿魏酸、香草酸和咖啡酸是天然药物中发现的酚酸。它们具有清除自由基和抑制血栓形成的生物活性。酚酸类化合物可抑制低密度脂蛋白的氧化，具有抗炎作用。本文首次建立了毛细管电泳联用化学发光法同时测定中药枸杞、双黄连口服液、蒲公英颗粒中4种酚酸的方法。毛细管电泳色谱柱为自组装毛细管电泳柱，未涂层熔融石英毛细管(有效长度66 cm，内径50μ)，背景电解液为3.0×10−5 M Ag(III)(pH=12.01)、3.0 mM鲁米诺(pH=9.20)和10 mM四硼酸钠溶液。进样时间为12 S(重力下)，分离电压为22kV。固相萃取(SPE)与CE-CL联用提高了灵敏度。在最佳条件下，绿原酸、阿魏酸、香草酸和咖啡酸的线性范围分别为0.625~20.0、1.000~30.0、0.150~1.5、0.045~1.00μg mL−1。方法的检出限为0.014~0.300μg mL−1，加标回收率为90.9%~119.8%，可用于中药中四种目标物质的测定。结果表明，所建立的方法是灵敏可靠的。并将该方法成功地应用于实际的中药材样品。
Chlorogenic, ferulic, vanillic, and caffeic acids are phenolic acids found in natural drugs. They possess the biological activities of scavenging free radicals and inhibiting thrombus formation. Phenolic acids can inhibit the oxidation of low-density lipoprotein, as well as have anti-inflammatory effects. This paper reports for the first time a capillary electrophoresis–chemiluminescence (CE–CL) method for the simultaneous determination of the four phenolic acids found in traditional and proprietary Chinese medicine, including Lycium chinense Miller, Shuanghuanglian oral liquid, and Taraxacum mongolicum granules. Capillary electrophoretic separation was performed on a self-assembled CE–CL device with an uncoated fused-silica capillary (66 cm effective length, 50 μm i.d.), and the background electrolyte was composed of 3.0 × 10−5 M Ag(iii) (pH = 12.01), 3.0 mM luminol (pH = 9.20), and 10 mM sodium tetraborate solution. The injection time was 12 s (under gravity) and the separation voltage was 22 kV. The combination of solid-phase extraction (SPE) and CE–CL improves the sensitivity. Under optimal conditions, calibration graphs displayed a linear range between 0.625 and 20.0, 1.000 and 30.0, 0.150 and 1.50, and 0.045 and 1.00 μg mL−1 for chlorogenic, ferulic, vanillic, and caffeic acid, respectively. The detection limit ranged from 0.014 to 0.300 μg mL−1. The practicality of using the proposed method to determine the four target analytes in traditional Chinese medicine was also validated, in which recoveries ranged from 90.9% to 119.8%. Taken together, these results indicate that the developed method is sensitive and reliable. Furthermore, the method was successfully applied to real traditional Chinese medicine samples.
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DOI：
10.1016/j.jchromb.2014.08.022
发表时间：
2014-10-15
影响因子：
3
作者：
Wang, Zhibing;Sun, Rui;Zhang, Hanqi
通讯作者：
Zhang, Hanqi
影响因子：
10.2
作者：
Shiozawa, Ryota;Inoue, Yutaka;Kanamoto, Ikuo
通讯作者：
Kanamoto, Ikuo
影响因子：
8.8
作者：
Liu, Shih-Chuan;Lin, Jau-Tien;Yang, Deng-Jye
通讯作者：
Yang, Deng-Jye
影响因子：
7.1
作者：
Johnston, KL;Clifford, MN;Morgan, LM
通讯作者：
Morgan, LM
金纳米颗粒毛细管电泳灵敏化学发光免疫分析
影响因子：
7.4
作者：
Liu, Yan-ming;Mei, Lin;Rei, Shu-wei
通讯作者：
Rei, Shu-wei