Ultrapure cyanogen bromide-cleaved glucagon: isolation in high yield by ion-exchange chromatography.
Ultrapure cyanogen bromide-cleaved glucagon: isolation in high yield by ion-exchange chromatography.
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超纯溴化氰裂解的胰高血糖素：通过离子交换色谱法高产率分离。
DOI：
10.1016/0003-9861(81)90085-0
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发表时间：
1981
影响因子：
3.9
通讯作者：
Gurd,RS
中科院分区：
文献类型：
--
作者：
Jones,BN;Gurd,RS
利用凝胶过滤和pH 4.5-5.2的阳离子交换层析，广泛地纯化了溴化氰裂解的胰高血糖素，产率为80-85%。在这个pH范围内，全息激素和它的裂解产物--截短的高丝氨酸内酯衍生物之间保持电荷差异，同时保持内酯环的完整性。纯度取决于缺少蛋氨酸和多肽水解后高丝氨酸的存在。高丝氨酸内酯在pH 9.5下用0.2三乙胺处理打开。内酯在室温下用三氟乙酸处理1h即可重整，但必须防止色氨酸-25的光氧化。高丝氨酸内酯形式与胰升糖素受体的结合不如高丝氨酸形式好。腺苷环化酶被内酯激活，其激活程度与天然激素相当，但浓度较高。所描述的步骤可用于提纯其他溴化氰裂解产物，并可用于基于溴化氰裂解的胰升糖素衍生物的半合成方法。
Cyanogen-bromide cleaved glucagon has been extensively purified in yields of 80–85% by the use of gel filtration and by cation-exchange chromatography at pH 4.5–5.2. This pH range maintains a charge difference between the holohormone and its cleavage product, the truncated homoserine lactone derivative, yet maintains the integrity of the lactone ring. Purity is determined by the lack of methionine and the presence of homoserine following peptide hydrolysis. The homoserine lactone is opened by treatment with 0.2ntriethylamine at pH 9.5. The lactone can be reformed by treatment with trifluoroacetic acid for 1 h at room temperature although protection against photooxidation of tryptophan-25 must be provided. The homoserine lactone form binds less well to glucagon receptors than does the homoserine form. Adenylate cyclase is activated by the lactone to an extent comparable to that obtained by native hormone but at elevated concentrations. The procedures described may be useful for purification of other cyanogen bromide cleavage products and is useful for semisynthetic methods based upon cyanogen bromide-cleaved derivatives of glucagon.
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影响因子：
2.9
作者：
LIN, MC;WRIGHT, DE;RODBELL, M
通讯作者：
RODBELL, M
DOI：
10.1016/0304-4165(71)90098-5
发表时间：
1971-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ASQUITH, RS;RIVETT, DE
通讯作者：
RIVETT, DE
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影响因子：
2.9
作者：
B. Jones;F. Dwulet;L. Lehman;M. Garner;R. Bogardt;W. Garner;F. Gurd
通讯作者：
F. Gurd
影响因子：
64.8
作者：
D. Dyckes;T. Creighton;R. Sheppard
通讯作者：
R. Sheppard
亚马逊河豚 (Inia geoffrensis) 主要成分肌红蛋白的完整氨基酸序列。
影响因子：
2.9
作者：
F. Dwulet;R. Bogardt;B. Jones;L. Lehman;F. Gurd
通讯作者：
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