Regulation of OsmiR156h through Alternative Polyadenylation Improves Grain Yield in Rice.
Regulation of OsmiR156h through Alternative Polyadenylation Improves Grain Yield in Rice.
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通过选择性多聚腺苷酸化调节 OsmiR156h 可提高水稻产量
DOI：
10.1371/journal.pone.0126154
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Zhao M;Liu B;Wu K;Ye Y;Huang S;Wang S;Wang Y;Han R;Liu Q;Fu X;Wu Y
为了养活不断增长的人口，需要大幅度提高谷类作物的产量。本研究表明，半矮高分蘖（SDT）的自然变异提高了水稻的收获指数和产量。功能获得性sdt突变在OsmiR156h microRNA前体上具有缩短的聚腺苷化尾部，导致OsmiR156h上调。携带sdt半显性等位基因的植株表现为株高降低，抗倒伏能力增强，单株分蘖数增加，籽粒产量增加。我们还发现，sdt等位基因与OsSPL14WFP等位基因组合可以有效地同时提高分蘖能力和穗支，从而提高收获指数和籽粒产量。最重要的是，在杂交水稻育种中，sdt等位基因和绿色革命基因sd1的金字塔化可使籽粒产量提高约20%。我们的研究结果表明，操纵OsmiR156的聚腺苷化状态代表了一种新的策略，可以提高水稻的产量潜力，而不是目前可以实现的。
Substantial increases in grain yield of cereal crops are required to feed a growing human population. Here we show that a natural variant of SEMIDWARF AND HIGH-TILLERING (SDT) increases harvest index and grain productivity in rice. Gain-of-function sdt mutation has a shortened polyadenylation tail on the OsmiR156h microRNA precursor, which cause the up-regulation of OsmiR156h. The plants carrying the semidominant sdt allele exhibit reduced plant height, enhanced lodging resistance, increased tiller numbers per plant, and resulting in an increased grain yield. We also show that combining the sdt allele with the OsSPL14WFP allele can be effective in simultaneously improving tillering capacity and panicle branching, thereby leading to higher harvest index and grain yield. Most importantly, pyramiding of the sdt allele and the green revolution gene sd1 enhances grain yield by about 20% in hybrid rice breeding. Our results suggest that the manipulation of the polyadenylation status of OsmiR156 represents a novel strategy for improving the yield potential of rice over what is currently achievable.
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