Experimental identification of Actinobacillus pleuropneumoniae strains L20 and JL03 heptosyltransferases, evidence for a new heptosyltransferase signature sequence.
Experimental identification of Actinobacillus pleuropneumoniae strains L20 and JL03 heptosyltransferases, evidence for a new heptosyltransferase signature sequence.
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DOI：
10.1371/journal.pone.0055546
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tomás JM
中科院分区：
文献类型：
--
作者：
Merino S;Knirel YA;Regué M;Tomás JM
We experimentally identified the activities of six predicted heptosyltransferases in Actinobacillus pleuropneumoniae genome serotype 5b strain L20 and serotype 3 strain JL03. The initial identification was based on a bioinformatic analysis of the amino acid similarity between these putative heptosyltrasferases with others of known function from enteric bacteria and Aeromonas. The putative functions of all the Actinobacillus pleuropneumoniae heptosyltrasferases were determined by using surrogate LPS acceptor molecules from well-defined A. hydrophyla AH-3 and A. salmonicida A450 mutants. Our results show that heptosyltransferases APL_0981 and APJL_1001 are responsible for the transfer of the terminal outer core D-glycero-D-manno-heptose (D,D-Hep) residue although they are not currently included in the CAZY glycosyltransferase 9 family. The WahF heptosyltransferase group signature sequence [S(T/S)(GA)XXH] differs from the heptosyltransferases consensus signature sequence [D(TS)(GA)XXH], because of the substitution of D261 for S261, being unique.
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影响因子：
3.2
作者：
Izquierdo, L;Coderch, N;Regué, M
通讯作者：
Regué, M
影响因子：
3.2
作者：
Jimenez, Natalia;Canals, Rocio;Tomas, Juan M.
通讯作者：
Tomas, Juan M.
影响因子：
3.1
作者：
Knirel, YA;Vinogradov, E;Tomás, JM
通讯作者：
Tomás, JM
影响因子：
3.3
作者：
Baltes, Nina;Buettner, Falk F. R.;Gerlach, Gerald-F.
通讯作者：
Gerlach, Gerald-F.
影响因子：
3.2
作者：
HITCHCOCK, PJ;BROWN, TM
通讯作者：
BROWN, TM