Preoperative Status of Gut Microbiota Predicts Postoperative Delirium in Patients With Gastric Cancer.
Preoperative Status of Gut Microbiota Predicts Postoperative Delirium in Patients With Gastric Cancer.
复制标题
术前肠道菌群状态可预测胃癌患者术后谵妄
DOI：
10.3389/fpsyt.2022.852269
复制
发表时间：
2022
影响因子：
4.7
通讯作者：
Liu XS
中科院分区：
文献类型：
--
作者：
Liu H;Cheng G;Xu YL;Fang Q;Ye L;Wang CH;Liu XS
术后妄想症(POD)是一种严重的术后并发症，尤其是老年腹部手术患者。越来越多的证据表明，肠道微生物区系与涉及“大脑-肠道”轴的心理障碍之间存在关联。然而，老年腹部手术后POD的发病机制与肠道微生物区系的组成之间的联系尚不清楚。40名接受腹部手术的患者(≥，65岁)被纳入研究。20例患者有POD，20例患者没有POD。术后应用混淆评估法(CAM)对POD进行诊断和评估。从所有患者的粪便样本中提取总DNA片段。16S rRNA测序确定肠道微生物区系组成。通过计算α-和β-多样性来确定样品的质量。α-和β-多样性表明，样品可以进行检测和比较。我们观察到患有POD和不患有POD的患者中存在多种不同丰富的细菌。一般情况下，变形杆菌、肠杆菌科、志贺氏菌、克雷伯氏菌、瘤胃球菌、玫瑰花、布鲁氏菌、霍尔德氏菌、厌氧菌、伯克霍尔德里亚科、消化球菌、乳杆菌和Dorea在POD队列中丰富，而在对照队列中马链球菌和人布鲁氏菌丰富。受试者工作特征(ROC)曲线分析结果表明，志贺氏菌的曲线下面积(AUC)为0.75。表型预测表明，肠道微生物区系可能通过改变对氧化应激的耐受性来影响POD。POD的发病机制与肠道微生物区系的组成有显著的相关性。志贺氏菌是预测老年人腹部手术后POD发病的有效诊断菌种。Http://www.chictr.org.cn/index.aspx，中国临床试验注册中心CHCTR200030131。
Post-operative delirium (POD) is a serious complication which occurs after surgery, especially in the elderly undergoing abdominal surgery. Increasing evidence has revealed an association between the gut microbiota and psychological disorders involving the “brain-gut” axis. However, the association between the pathogenesis of POD after abdominal surgery in aging and composition of the gut microbiota remains unclear. Forty patients (≥65 years old) who underwent abdominal surgery were included in the study. Twenty patients had POD, whereas 20 patients did not. POD was diagnosed and assessed using the confusion assessment method (CAM) during the postoperative period. Total DNA fractions were extracted from all fecal samples of patients. 16S rRNA sequencing was performed to determine the composition of the gut microbiota. The quality of the samples was determined by calculating the α- and β-diversities. The α- and β-diversities indicated that the samples were eligible for detection and comparison. We observed multiple differentially abundant bacteria in patients with and without POD. Generally, Proteobacteria, Enterbacteriaceae, Escherichia shigella, Klebsiella, Ruminococcus, Roseburia, Blautia, Holdemanella, Anaerostipes, Burkholderiaceae, Peptococcus, Lactobacillus, and Dorea were abundant in the POD cohort, whereas Streptococcus equinus and Blautia hominis were abundant in the control cohort. The results of receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) of Escherichia shigella was 0.75. Phenotype prediction showed that the gut microbiota may influence POD by altering the tolerance to oxidative stress. There were significant associations between the pathogenesis of POD and composition of the gut microbiota. Escherichia shigella are promising diagnostic bacterial species for predicting POD onset after abdominal surgery in elderly people. http://www.chictr.org.cn/index.aspx, Chinese Clinical Trial Registry ChiCTR200030131.
登录
查看更多内容
DOI：
10.1016/j.athoracsur.2015.12.074
发表时间：
2016-05
期刊：
The Annals of thoracic surgery
影响因子：
--
作者：
Brown CH 4th;Laflam A;Max L;Lymar D;Neufeld KJ;Tian J;Shah AS;Whitman GJ;Hogue CW
通讯作者：
Hogue CW
影响因子：
5.9
作者：
Megur A;Baltriukienė D;Bukelskienė V;Burokas A
通讯作者：
Burokas A
影响因子：
3.7
作者：
Raats JW;van Eijsden WA;Crolla RM;Steyerberg EW;van der Laan L
通讯作者：
van der Laan L
影响因子：
168.9
作者：
Inouye, Sharon K.;Westendorp, Rudi G. J.;Saczynski, Jane S.
通讯作者：
Saczynski, Jane S.
影响因子：
3.9
作者：
Bickford PC;Flowers A;Grimmig B
通讯作者：
Grimmig B