Self-collection of capillary blood using Tasso-SST devices for Anti-SARS-CoV-2 IgG antibody testing.
Self-collection of capillary blood using Tasso-SST devices for Anti-SARS-CoV-2 IgG antibody testing.
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DOI：
10.1371/journal.pone.0255841
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Morishima C
中科院分区：
文献类型：
--
作者：
Hendelman T;Chaudhary A;LeClair AC;van Leuven K;Chee J;Fink SL;Welch EJ;Berthier E;Quist BA;Wald A;Wener MH;Hoofnagle AN;Morishima C
在当前的SARS-CoV-2大流行期间，为尽量减少COVID-19暴露所做的努力导致了获得医疗服务和检测的限制。Tasso-SST试剂盒包括远程毛细管血液自我采集所需的所有组件。在本研究中，我们试图探讨Tasso-SST装置作为测量SARS-CoV-2 IgG抗体的自收集装置的准确性和可靠性。采用无监督和有监督Tasso-SST装置采集毛细血管血，采用标准静脉穿刺采集静脉血。在测试之前，无人监督的自行采集的血液样本经历了极端的夏季或冬季模拟运输条件。在clia认证的临床实验室使用euroimmune抗sars - cov -2 S1 IgG检测同时检测所有三种方法获得的血清。无监督和有监督的Tasso-SST毛细血管采血成功率分别为93.4%和94.5%。然后对56名参与者，55名记录的（PCR+） COVID-19患者和33名健康对照者的血清进行抗sars - cov -2 IgG抗体检测。与静脉血结果相比，tasso - sst采集（无应激）和夏季和冬季应激血液样品的Deming回归斜率分别为1.00 （95% CI: 0.99 - 1.02）、1.00 （95% CI: 0.98-1.01）和0.99 (95% CI: 0.97-1.01)，总体准确率为98.9%。采用Tasso-SST装置进行毛细管自采成功率高。此外，Tasso-SST毛细血管与标准静脉血源血清抗sars - cov -2 IgG结果具有良好的一致性。Tasso-SST装置应该能够在没有医疗监督的情况下广泛采集毛细血管血液进行检测，促进流行病学研究。
Efforts to minimize COVID-19 exposure during the current SARS-CoV-2 pandemic have led to limitations in access to medical care and testing. The Tasso-SST kit includes all of the components necessary for remote, capillary blood self-collection. In this study, we sought to investigate the accuracy and reliability of the Tasso-SST device as a self-collection device for measurement of SARS-CoV-2 IgG antibodies. Capillary blood was obtained via unsupervised and supervised application of the Tasso-SST device, and venous blood was collected by standard venipuncture. Unsupervised self-collected blood samples underwent either extreme summer or winter-simulated shipping conditions prior to testing. Sera obtained by all three methods were tested concurrently using the EuroImmun anti-SARS-CoV-2 S1 IgG assay in a CLIA-certified clinical laboratory. Successful Tasso-SST capillary blood collection by unsupervised and supervised administration was completed by 93.4% and 94.5% of participants, respectively. Sera from 56 participants, 55 with documented (PCR+) COVID-19, and 33 healthy controls were then tested for anti-SARS-CoV-2 IgG antibodies. Compared to venous blood results, Tasso-SST-collected (unstressed) and the summer- and winter-stressed blood samples demonstrated Deming regression slopes of 1.00 (95% CI: 0.99–1.02), 1.00 (95% CI: 0.98–1.01), and 0.99 (95% CI: 0.97–1.01), respectively, with an overall accuracy of 98.9%. Capillary blood self-collection using the Tasso-SST device had a high success rate. Moreover, excellent concordance was found for anti-SARS-CoV-2 IgG results between Tasso-SST capillary and standard venous blood-derived sera. The Tasso-SST device should enable widespread collection of capillary blood for testing without medical supervision, facilitating epidemiologic studies.
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DOI：
10.15585/mmwr.mm6943a3
发表时间：
2020-10-30
期刊：
MMWR. Morbidity and mortality weekly report
影响因子：
--
作者：
Koonin LM;Hoots B;Tsang CA;Leroy Z;Farris K;Jolly T;Antall P;McCabe B;Zelis CBR;Tong I;Harris AM
通讯作者：
Harris AM