PRINT: a novel platform toward shape and size specific nanoparticle theranostics.
PRINT: a novel platform toward shape and size specific nanoparticle theranostics.
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DOI：
10.1021/ar2000315
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发表时间：
2011-10-18
影响因子：
18.3
通讯作者：
Desimone, Joseph M.
中科院分区：
文献类型：
--
作者：
Perry, Jillian L.;Herlihy, Kevin P.;Napier, Mary E.;Desimone, Joseph M.
纳米诊断学是融合纳米技术、治疗学和诊断学的新一代医学。通过将治疗和显像剂整合到一个纳米颗粒中，这种新的治疗策略不仅具有检测和诊断疾病的潜力，而且还具有治疗和监测治疗反应的潜力。这种能力可以在研究环境和临床环境中产生深远的影响。在研究环境中，这样的能力将允许研究科学家快速评估新疗法的性能，努力迭代他们的设计，以提高治疗指数和疗效。在临床环境中，治疗学根据与被测试的生物学机制相关的假设，提供了确定参加临床试验的患者是否对特定治疗有反应或预期有反应的能力。如果没有，患者可以更快地退出临床试验，转向其他治疗方案。为了有效，这些治疗药物必须具有高度的部位特异性。最理想的情况是，它们将携带相关货物，演示货物的受控释放，并包括具有高信噪比的成像探头。人体中存在许多生物屏障，对纳米颗粒递送载体的有效性提出了挑战。这些屏障包括但不限于血管壁、颗粒在器官中的物理滞留以及吞噬细胞对颗粒的清除。在全身给药过程中，循环颗粒的快速清除是一个主要挑战；目前的研究试图确定控制纳米载体性能的关键设计参数，如尺寸、表面化学、弹性和形状。颗粒大小和表面化学对纳米载体体内生物分布的影响已被广泛研究，并建立了一般准则。最近有文献表明，形状和弹性可以对运载工具的行为产生深远的影响。因此，具有独立控制形状、尺寸、基质、表面化学和模量的能力对于设计成功的递送剂至关重要。在本帐户中，我们描述了在非润湿模板（PRINT）中使用颗粒复制来制造形状和尺寸特定的微粒和纳米颗粒。PRINT方法的一个特殊优点是它可以精确控制形状、尺寸、表面化学和模量。我们已经演示了用化疗药物、磁共振造影剂和荧光团装载PRINT颗粒。PRINT颗粒的表面特性可以很容易地用“隐形”聚（乙二醇）链进行修饰，以增加血液循环时间，用靶向部分进行靶向递送或用放射性标签进行核成像。这些粒子在纳米医学和诊断方面具有巨大的应用潜力。
Nanotheranostics represents the next generation of medicine, fusing nanotechnology, therapeutics, and diagnostics. By integrating therapeutic and imaging agents into one nanoparticle, this new treatment strategy has the potential not only to detect and diagnose disease but also to treat and monitor the therapeutic response. This capability could have a profound impact in both the research setting as well as in a clinical setting. In the research setting, such a capability will allow research scientists to rapidly assess the performance of new therapeutics in an effort to iterate their designs for increased therapeutic index and efficacy. In the clinical setting, theranostics offers the ability to determine whether patients enrolling in clinical trials are responding, or are expected to respond, to a given therapy based on the hypothesis associated with the biological mechanisms being tested. If not, patients can be more quickly removed from the clinical trial and shifted to other therapeutic options. To be effective, these theranostic agents must be highly site specific. Optimally, they will carry relevant cargo, demonstrate controlled release of that cargo, and include imaging probes with a high signal-to-noise ratio. There are many biological barriers in the human body that challenge the efficacy of nanoparticle delivery vehicles. These barriers include, but are not limited to, the walls of blood vessels, the physical entrapment of particles in organs, and the removal of particles by phagocytic cells. The rapid clearance of circulating particles during systemic delivery is a major challenge; current research seeks to define key design parameters that govern the performance of nanocarriers, such as size, surface chemistry, elasticity, and shape. The effect of particle size and surface chemistry on in vivo biodistribution of nanocarriers has been extensively studied, and general guidelines have been established. Recently it has been documented that shape and elasticity can have a profound effect on the behavior of delivery vehicles. Thus, having the ability to independently control shape, size, matrix, surface chemistry, and modulus is crucial for designing successful delivery agents. In this Account, we describe the use of particle replication in nonwetting templates (PRINT) to fabricate shape- and size-specific microparticles and nanoparticles. A particular strength of the PRINT method is that it affords precise control over shape, size, surface chemistry, and modulus. We have demonstrated the loading of PRINT particles with chemotherapeutics, magnetic resonance contrast agents, and fluorophores. The surface properties of the PRINT particles can be easily modified with “stealth” poly(ethylene glycol) chains to increase blood circulation time, with targeting moieties for targeted delivery or with radiolabels for nuclear imaging. These particles have tremendous potential for applications in nanomedicine and diagnostics.
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由于异常高的化学治疗载荷，有效的工程PLGA纳米颗粒。
影响因子：
10.8
作者：
Enlow EM;Luft JC;Napier ME;DeSimone JM
通讯作者：
DeSimone JM
影响因子：
4.9
作者：
Alexis F;Pridgen E;Molnar LK;Farokhzad OC
通讯作者：
Farokhzad OC
影响因子：
18.3
作者：
Gratton, Stephanie E. A.;Williams, Stuart S.;Napier, Mary E.;Pohlhaus, Patrick D.;Zhou, Zhilian;Wiles, Kenton B.;Maynor, Benjamin W.;Shen, Clifton;Olafsen, Tove;Samulski, Edward T.;Desimone, Joseph M.
通讯作者：
Desimone, Joseph M.
DOI：
10.1002/wnan.40
发表时间：
2009-07
影响因子：
8.6
作者：
Canelas, Dorian A.;Herlihy, Kevin P.;DeSimone, Joseph M.
通讯作者：
DeSimone, Joseph M.
影响因子：
6.6
作者：
Caldorera-Moore M;Guimard N;Shi L;Roy K
通讯作者：
Roy K