TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana.
TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana.
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DOI：
10.3389/fpls.2015.00108
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发表时间：
2015
影响因子：
5.6
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Wang F;Lin R;Feng J;Chen W;Qiu D;Xu S
植物特异性NAC转录因子（phytospecific NAC transcription factors, TFs）是一个大家族，在调控植物发育过程和对环境胁迫的响应中发挥重要作用，但目前仅有部分NAC转录因子在谷物病害反应中的作用被研究。病毒诱导的基因沉默（VIGS）是快速分析植物组织中基因功能的有效策略。本研究从面包小麦中克隆了编码NAC1亚群新成员的TaNAC1，并对其进行了鉴定。它是一种定位于细胞核的TF，在其c端含有一个激活域。TaNAC1在小麦根系中强烈表达，并参与对专性病原菌小麦纹状锈菌（Puccinia striiformis f. sp. tritici）侵染和防御相关激素处理（如水杨酸、茉莉酸甲酯和乙烯）的响应。用大麦条纹花叶病毒诱导的基因沉默（BSMV-VIGS）敲除TaNAC1增强了小麦的条纹锈病抗性。tanac1在拟南芥中的过表达增强了植物对丁香假单胞菌DC3000的敏感性，减弱了系统获得性抗性，促进了侧根的发育。茉莉酸信号通路基因PDF1.2和ORA59在转基因植株中组成性表达。TaNAC1过表达抑制了与SA信号通路相关的抗性基因PR1和PR2以及作为JA和SA信号通路连接节点的AtWRKY70的表达水平。总的来说，TaNAC1是NAC1亚群中的一个新的NAC成员，负调控植物抗病性，并可能调节植物JA-和sa -信号防御级联反应。
Plant-specific NAC transcription factors (TFs) constitute a large family and play important roles in regulating plant developmental processes and responses to environmental stresses, but only some of them have been investigated for effects on disease reaction in cereal crops. Virus-induced gene silencing (VIGS) is an effective strategy for rapid functional analysis of genes in plant tissues. In this study, TaNAC1, encoding a new member of the NAC1 subgroup, was cloned from bread wheat and characterized. It is a TF localized in the cell nucleus, and contains an activation domain in its C-terminal. TaNAC1 was strongly expressed in wheat roots and was involved in responses to infection by the obligate pathogen Puccinia striiformis f. sp. tritici and defense-related hormone treatments such as salicylic acid (SA), methyl jasmonate, and ethylene. Knockdown of TaNAC1 with barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) enhanced stripe rust resistance. TaNAC1-overexpression in Arabidopsis thaliana plants gave enhanced susceptibility, attenuated systemic-acquired resistance to Pseudomonas syringae DC3000, and promoted lateral root development. Jasmonic acid-signaling pathway genes PDF1.2 and ORA59 were constitutively expressed in transgenic plants. TaNAC1 overexpression suppressed the expression levels of resistance-related genes PR1 and PR2 involved in SA signaling and AtWRKY70, which functions as a connection node between the JA- and SA-signaling pathways. Collectively, TaNAC1 is a novel NAC member of the NAC1 subgroup, negatively regulates plant disease resistance, and may modulate plant JA- and SA-signaling defense cascades.
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影响因子：
7.2
作者：
Hao, Yu-Jun;Wei, Wei;Chen, Shou-Yi
通讯作者：
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影响因子：
4.3
作者：
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通讯作者：
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影响因子：
14.9
作者：
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影响因子：
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作者：
Kaneda, Takashi;Taga, Yuri;Che, Fang-Sik
通讯作者：
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影响因子：
7.2
作者：
Berrocal-Lobo, M;Molina, A;Solano, R
通讯作者：
Solano, R