Biological effects of asbestos fibers on human cells in vitro--especially on lymphocytes and neutrophils.
Biological effects of asbestos fibers on human cells in vitro--especially on lymphocytes and neutrophils.
复制标题
石棉纤维对体外人体细胞的生物效应，特别是对淋巴细胞和中性粒细胞的影响。
作者：
A. Ueki
本文综述了石棉纤维的生物学效应，包括对人淋巴细胞的多克隆活化作用和对人中性粒细胞释放自由基的影响。温石棉、青石棉和铁石棉多克隆激活CD 4 + T淋巴细胞，随后激活诱导细胞死亡（一种细胞凋亡）。活化是HLA II类依赖性的，并且在已被石棉纤维刺激的具有高Ca++水平的分级分离的T细胞中检测到某些V β库，例如V β 5.3。这些观察结果支持石棉作为超抗原的可能性，石棉在体内反复刺激淋巴细胞。研究表明，超氧阴离子自由基和羟自由基清除剂可以抑制石棉的细胞毒性。这些清除剂中的一些如二甲基亚砜（DMSO）或视黄酸已知为细胞分化的诱导剂。DMSO诱导HL-60细胞向中性粒细胞分化的生物学功能被青石棉抑制，这是因为DMSO与青石棉刺激后释放的羟基自由基反应并消耗自身。超氧化物歧化酶（SOD）抑制青石棉的作用，在 *OH二次释放之前迅速与 *O2-反应。石棉纤维，特别是青石棉，可能通过释放自由基和消耗作为自由基清除剂的细胞分化诱导剂来抑制组织细胞分化。
Biological effects of asbestos fibers were reviewed in relation to the polyclonal activation of human lymphocytes and to the release of free radicals from human neutrophils in vitro. Chrysotile, crocidolite, and amosite asbestos activate CD4+ T lymphocytes polyclonally, followed by activation-induced cell death (a type of apoptosis). The activation is HLA class II dependent, and certain Vbeta repertoire, e.g. Vbeta 5.3, are detected among the fractionated T cells with a high Ca++ level that had been stimulated by asbestos fibers. These observations support the possibility that asbestos acts as a superantigen, and that asbestos stimulate lymphocytes repeatedly in vivo. It has been reported that asbestos-induced cytotoxicity can be suppressed by the scavengers of superoxide or hydroxyl radical. Some of these scavengers such as dimethylsulfoxide (DMSO) or retinoic acid are known as inducers of cell differentiation. The biological functions of DMSO for cell differentiation of HL-60 cells to neutrophils are suppressed by co-culturing of crocidolite asbestos, because DMSO reacts with the hydroxyl radical released after the stimulation with crocidolite and spent itself. Superoxide dismutase (SOD) inhibited the effects of crocidolite, reacting rapidly with *O2- before the secondary release of *OH. It seems to be probable that asbestos fibers, especially crocidolite, suppress the tissue cell differentiation by releasing free radicals and by wasting inducers of cell differentiation as radical scavengers.
登录
查看更多内容
影响因子：
4.4
作者：
C. Weyand;J. Goronzy;C. Fathman
通讯作者：
C. Weyand;J. Goronzy;C. Fathman
暴露于石棉状和非纤维颗粒的肺泡巨噬细胞产生超氧化物（O2-）。
影响因子：
11.2
作者：
Hansen,K;Mossman,BT
通讯作者：
Mossman,BT
CD8 分子与细胞毒性 T 细胞上的 T 细胞受体复合物之间存在关联的证据。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Takada,S;Engleman,EG
通讯作者：
Engleman,EG
石棉诱导的人淋巴细胞有丝分裂反应的改变。
影响因子：
8.3
作者：
Bozelka,BE;Gaumer,HR;Nordberg,J;Salvaggio,JE
通讯作者：
Salvaggio,JE
免疫化学证据表明，在二甲亚砜和视黄酸诱导的 HL-60 分化过程中，三种蛋白激酶 C 同工酶丰度增加。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Makowske,M;Ballester,R;Cayre,Y;Rosen,OM
通讯作者：
Rosen,OM