Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector
Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector
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通过在遗传不稳定载体上瞬时表达 Cre 来回收非常规酵母 Xanthophylllomyces dendrorhous 中的选择性标记
DOI：
10.1007/s00253-018-9496-1
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发表时间：
2018-12
影响因子：
5
通讯作者：
Wang Shi'an
中科院分区：
文献类型：
--
作者：
Zhang Ning;Li Jiaxin;Li Fuli;Wang Shi'an
选择标记回收是生物工程中的一项基本技术。然而，这种技术通常在非模式微生物中不可用。在这项研究中，我们提出了一个简单而有效的方法，选择标记回收的虾青素合成酵母Xanthophyllomyces dendrohous。该方法基于Cre-loxP系统，其中Cre重组酶的瞬时表达由独立于附加型质粒和诱导型启动子的遗传不稳定载体控制。单基因位点和多基因位点的选择标记随着Cre载体的丢失而沿着去除，去除率分别为100%和29%。该方法的重要性突出的发现，稳定的自养突变体是不容易获得的X。树枝状的X.树状结构和非同源末端连接优势酵母Yarrowia lipolytica表明，该方法可普遍用于同源重组优势酵母。
Selectable marker recycling is a basic technique in bioengineering. However, this technique is usually unavailable in non-model microorganisms. In this study, we proposed a simple and efficient method for selectable marker recycling in the astaxanthin-synthesizing yeast Xanthophyllomyces dendrorhous. This method was based on a Cre-loxP system, in which the transient expression of the Cre recombinase was controlled by a genetically unstable vector independent of episomal plasmids and inducible promoters. The selectable markers in single-gene locus and multigene loci were removed along with the loss of the Cre vector with a ratio of 100% and 29%, respectively. The significance of the method was highlighted by the finding that stable autotrophic mutants were not readily obtained in X. dendrorhous. Comparative studies in X. dendrorhous and the non-homologous end joining dominant yeast Yarrowia lipolytica suggested that the method could be universally used in homologous recombination dominant yeasts.
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影响因子：
2.2
作者：
Weidong Qian;Hao Song;Yueyong Liu;Chaozheng Zhang;Zhendong Niu;Hui Wang;B. Qiu
通讯作者：
Weidong Qian;Hao Song;Yueyong Liu;Chaozheng Zhang;Zhendong Niu;Hui Wang;B. Qiu
影响因子：
2.6
作者：
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通讯作者：
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影响因子：
4.1
作者：
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通讯作者：
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影响因子：
3
作者：
Dennison, PMJ;Ramsdale, M;Brown, AJP
通讯作者：
Brown, AJP