Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stress.
Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stress.
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DOI：
10.1186/1471-2229-14-56
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发表时间：
2014-03-04
影响因子：
5.3
通讯作者：
Jordano J
中科院分区：
文献类型：
--
作者：
Personat JM;Tejedor-Cano J;Prieto-Dapena P;Almoguera C;Jordano J
我们以前曾报道，种子特异性过表达的向日葵（向日葵L.）热激因子A9（HaHSFA9）增强转基因烟草（Nicotiana tabacum L.）的种子寿命。此外，HaHSFA9在营养器官中的过表达赋予了对剧烈水平的脱水和氧化应激的耐受性。在这里，我们发现向日葵热休克因子A4 a（HaHSFA 4a）和HaHSFA 9的联合过表达增强了之前报道的单独HaHSFA 9过表达所描述的所有表型。表型的改善与HaHSFA9激活的基因编码的小热休克蛋白（sHSP）积累的微小变化一致。在控制生长条件下，营养器官（缺乏内源性HSFA9蛋白）中HaHSFA4a的单一过表达不会诱导sHSP积累，也不会赋予耐热性。HaHSFA4a单独过表达也不能诱导对严重非生物胁迫的耐受性。因此，这两个因素的协同作用在幼苗中是明显的。我们的研究表明HaHSFA4a需要HaHSFA9才能在植物体内发挥功能。我们的研究结果强烈支持HaHSFA4a和HaHSFA9参与转录共激活的遗传程序的寿命和耐脱水向日葵种子。这些结果对提高种子寿命和营养器官对严重胁迫的耐受性具有潜在的应用价值。
We have previously reported that the seed-specific overexpression of sunflower (Helianthus annuus L.) Heat Shock Factor A9 (HaHSFA9) enhanced seed longevity in transgenic tobacco (Nicotiana tabacum L.). In addition, the overexpression of HaHSFA9 in vegetative organs conferred tolerance to drastic levels of dehydration and oxidative stress. Here we found that the combined overexpression of sunflower Heat Shock Factor A4a (HaHSFA4a) and HaHSFA9 enhanced all the previously reported phenotypes described for the overexpression of HaHSFA9 alone. The improved phenotypes occurred in coincidence with only subtle changes in the accumulation of small Heat Shock Proteins (sHSP) that are encoded by genes activated by HaHSFA9. The single overexpression of HaHSFA4a in vegetative organs (which lack endogenous HSFA9 proteins) did not induce sHSP accumulation under control growth conditions; neither it conferred thermotolerance. The overexpression of HaHSFA4a alone also failed to induce tolerance to severe abiotic stress. Thus, a synergistic functional effect of both factors was evident in seedlings. Our study revealed that HaHSFA4a requires HaHSFA9 for in planta function. Our results strongly support the involvement of HaHSFA4a and HaHSFA9 in transcriptional co-activation of a genetic program of longevity and desiccation tolerance in sunflower seeds. These results would also have potential application for improving seed longevity and tolerance to severe stress in vegetative organs.
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影响因子：
3.7
作者：
Li Z;Zhang L;Wang A;Xu X;Li J
通讯作者：
Li J
影响因子：
4.6
作者：
Hardtke, CS;Ckurshumova, W;Berleth, T
通讯作者：
Berleth, T
影响因子：
4.8
作者：
Almoguera, C;Rojas, A;Jordano, J
通讯作者：
Jordano, J
DOI：
10.1007/s004380050731
发表时间：
1998-05-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Prändl, R;Hinderhofer, K;Schöffl, F
通讯作者：
Schöffl, F
影响因子：
11.6
作者：
Davletova, S;Rizhsky, L;Mittler, R
通讯作者：
Mittler, R