Ectopic overexpression of SlHsfA3, a heat stress transcription factor from tomato, confers increased thermotolerance and salt hypersensitivity in germination in transgenic Arabidopsis.
Ectopic overexpression of SlHsfA3, a heat stress transcription factor from tomato, confers increased thermotolerance and salt hypersensitivity in germination in transgenic Arabidopsis.
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DOI：
10.1371/journal.pone.0054880
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Li Z;Zhang L;Wang A;Xu X;Li J
植物热胁迫转录因子（Hsfs）是介导植物对各种环境胁迫反应的关键成分。然而，许多植物hsf的详细作用还远未完全了解。本研究从栽培番茄（Solanum lycopersicum, Sl）中分离到一株Hsf (SlHsfA3)，并在遗传和发育水平上进行了功能鉴定。核定位的SlHsfA3在植物各器官中基本且普遍表达。热胁迫对SlHsfA3的表达有显著的诱导作用，高盐胁迫对SlHsfA3的表达有中等的诱导作用，干旱胁迫对SlHsfA3的表达有轻微的诱导作用，而ABA对SlHsfA3的表达没有诱导作用。SlHsfA3异位过表达增加了转基因拟南芥植株的耐热性和晚开花表型。此外，SlHsfA3在盐胁迫下对种子萌发有负调控作用。rna测序数据表明，在过表达SlHsfA3的拟南芥植物中诱导了许多热休克蛋白（Hsps）和胁迫相关基因。凝胶转移实验和瞬时表达分析表明，SlHsfA3直接激活SlHsp26.1-P和SlHsp21.5-ER的表达。综上所述，我们的研究结果表明，SlHsfA3作为一种典型的Hsf，对植物的耐热性有贡献。来自SlHsfA3过表达系的晚开花和种子萌发表型以及RNA-seq数据进一步证实了植物Hsfs参与与不利条件相关的多种生理生化过程的假设。
Plant heat stress transcription factors (Hsfs) are the critical components involved in mediating responses to various environmental stressors. However, the detailed roles of many plant Hsfs are far from fully understood. In this study, an Hsf (SlHsfA3) was isolated from the cultivated tomato (Solanum lycopersicum, Sl) and functionally characterized at the genetic and developmental levels. The nucleus-localized SlHsfA3 was basally and ubiquitously expressed in different plant organs. The expression of SlHsfA3 was induced dramatically by heat stress, moderately by high salinity, and slightly by drought, but was not induced by abscisic acid (ABA). The ectopic overexpression of SlHsfA3 conferred increased thermotolerance and late flowering phenotype to transgenic Arabidopsis plants. Moreover, SlHsfA3 played a negative role in controlling seed germination under salt stress. RNA-sequencing data demonstrated that a number of heat shock proteins (Hsps) and stress-associated genes were induced in Arabidopsis plants overexpressing SlHsfA3. A gel shift experiment and transient expression assays in Nicotiana benthamiana leaves demonstrated that SlHsfA3 directly activates the expression of SlHsp26.1-P and SlHsp21.5-ER. Taken together, our results suggest that SlHsfA3 behaves as a typical Hsf to contribute to plant thermotolerance. The late flowering and seed germination phenotypes and the RNA-seq data derived from SlHsfA3 overexpression lines lend more credence to the hypothesis that plant Hsfs participate in diverse physiological and biochemical processes related to adverse conditions.
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影响因子：
7.4
作者：
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通讯作者：
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DOI：
10.1016/j.bbrc.2010.09.038
发表时间：
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影响因子：
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作者：
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