Age-dependent changes in autophosphorylation of alpha calcium/calmodulin dependent kinase II in hippocampus and amygdala after contextual fear conditioning.
Age-dependent changes in autophosphorylation of alpha calcium/calmodulin dependent kinase II in hippocampus and amygdala after contextual fear conditioning.
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DOI：
10.1016/j.brainresbull.2017.06.012
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发表时间：
2017-09
影响因子：
3.8
通讯作者：
Giese KP
中科院分区：
文献类型：
--
作者：
Fang T;Kasbi K;Rothe S;Aziz W;Giese KP
条件反射诱导的CA 3区磷酸化-α CaMK Ⅱ的增加具有年龄依赖性。老化增加了CA 3区磷/总αCaMKII的比率。条件反射诱导的外侧杏仁核αCaMKII减少具有年龄依赖性衰老对杏仁核中央和基底外侧的α CaMK Ⅱ无影响。海马体和杏仁核是负责情境恐惧条件反射（CFC）的重要脑区。α钙-钙调蛋白激酶II（αCaMKII）在苏氨酸-286（T286）处的自磷酸化是涉及长时程增强（LTP）、学习和记忆的关键步骤。然而，αCaMKII水平随年龄增长和相关大脑区域训练的变化尚未完全了解。在这里，我们研究了老化和训练如何影响海马和杏仁核中磷酸化（T286）水平和磷酸化：总αCaMKII的比例。通过免疫组织化学法分析幼年和老年小鼠（未经训练和在CFC中训练）海马和杏仁核中总αCaMKII和磷酸化αCaMKII的水平。我们发现CFC训练后2小时，年轻小鼠海马CA 3放射层中磷酸化水平较高，磷酸化/总α CaMK II的比例增加。此外，与年轻的未训练组相比，老年未训练小鼠海马CA 3区的磷酸化：总αCaMKII比率更高。在杏仁核的中央、外侧和基底外侧区域，对于磷酸化和磷酸化αCaMKII与总αCaMKII的比率，均未观察到训练或老化的影响。这些结果表明，老化损害了海马CA 3放射层中训练诱导的自磷酸化（T286）α CaMK II上调。这表明，不同的年龄相关机制的基础CFC可能更严重地依赖于NMDA受体依赖的可塑性在年轻的时候。
Conditioning-induced increase of phospho-αCaMKII in area CA3 is age-dependent. Aging increases the ratio of phosphor/total αCaMKII in area CA3. Conditioning-induced decrease of αCaMKII in lateral amygdala is age-dependent. Aging does not impact on αCaMKII in central and basolateral amygdala. The hippocampus and amygdala are essential brain regions responsible for contextual fear conditioning (CFC). The autophosphorylation of alpha calcium-calmodulin kinase II (αCaMKII) at threonine-286 (T286) is a critical step implicated in long-term potentiation (LTP), learning and memory. However, the changes in αCaMKII levels with aging and training in associated brain regions are not fully understood. Here, we studied how aging and training affect the levels of phosphorylated (T286) and proportion of phosphorylated:total αCaMKII in the hippocampus and amygdala. Young and aged mice, naïve (untrained) and trained in CFC, were analysed by immunohistochemistry for the levels of total and phosphorylated αCaMKII in the hippocampus and amygdala. We found that two hours after CFC training, young mice exhibited a higher level of phosphorylated and increased ratio of phosphorylated:total αCaMKII in hippocampal CA3 stratum radiatum. Furthermore, aged untrained mice showed a higher ratio of phosphorylated:total αCaMKII in the CA3 region of the hippocampus when compared to the young untrained group. No effect of training or aging were seen in the central, lateral and basolateral amygdala regions, for both phosphorylated and ratio of phosphorylated:total αCaMKII. These results show that aging impairs the training-induced upregulation of autophosphorylated (T286) αCaMKII in the CA3 stratum radiatum of the hippocampus. This indicates that distinct age-related mechanisms underlie CFC that may rely more heavily on NMDA receptor-dependent plasticity in young age.
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影响因子：
34.7
作者：
Lisman, John;Yasuda, Ryohei;Raghavachari, Sridhar
通讯作者：
Raghavachari, Sridhar
影响因子：
3.5
作者：
MOORE, CI;BROWNING, MD;ROSE, GM
通讯作者：
ROSE, GM
DOI：
10.1523/jneurosci.2036-08.2008
发表时间：
2008-08-06
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Boric K;Muñoz P;Gallagher M;Kirkwood A
通讯作者：
Kirkwood A
DOI：
10.1016/j.mcn.2015.08.003
发表时间：
2015-09
期刊：
Molecular and cellular neurosciences
影响因子：
--
作者：
Pasek JG;Wang X;Colbran RJ
通讯作者：
Colbran RJ
DOI：
10.1073/pnas.1109680108
发表时间：
2011-11-08
影响因子：
11.1
作者：
Radwanska, Kasia;Medvedev, Nikolay I.;Giese, K. Peter
通讯作者：
Giese, K. Peter