Brain levels of prostaglandins, endocannabinoids, and related lipids are affected by mating strategies.
Brain levels of prostaglandins, endocannabinoids, and related lipids are affected by mating strategies.
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DOI：
10.1155/2013/436252
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发表时间：
2013
影响因子：
2.8
通讯作者：
Bradshaw HB
中科院分区：
文献类型：
--
作者：
Stuart JM;Paris JJ;Frye C;Bradshaw HB
背景。内源性大麻素（eCBs）参与生殖行为的发育和调节。同样，前列腺素（pg）驱动性别分化和排卵的启动。在这里，我们使用脂质组学策略来测试交配立即激活eCBs和pg的生物合成和/或代谢的假设，以及特定的交配策略对大脑中这些脂质的差异调节。方法。脂质提取和串联质谱分析对经历过两种交配策略（节奏或标准交配）之一和两种非交配组（室内暴露和家庭笼控制）的发情大鼠的大脑进行了分析。测定嗅球、下丘脑、海马、丘脑、纹状体、中脑、小脑和脑干中PGs （PGE2和PGF2alpha）、eCBs （AEA和2-AG、n -花生四烯醇酰甘氨酸）和4种相关脂质（4种n -酰基乙醇酰胺）的水平。结果。总体而言，与标准交配大鼠相比，这些脂质水平显著降低，脑干、海马体、中脑和纹状体的降幅最大。然而，与家养笼子相比，暴露在室内的大鼠具有明显更高的这些脂质水平，其中海马体显示出最大的增长。结论。这些数据表明，交配策略和暴露于交配场所会影响大脑中的脂质信号。
Background. Endogenous cannabinoids (eCBs) are involved in the development and regulation of reproductive behaviors. Likewise, prostaglandins (PGs) drive sexual differentiation and initiation of ovulation. Here, we use lipidomics strategies to test the hypotheses that mating immediately activates the biosynthesis and/or metabolism of eCBs and PGs and that specific mating strategies differentially regulate these lipids in the brain. Methods. Lipid extractions and tandem mass spectrometric analysis were performed on brains from proestrous rats that had experienced one of two mating strategies (paced or standard mating) and two nonmated groups (chamber exposed and home cage controls). Levels of PGs (PGE2 and PGF2alpha), eCBs (AEA and 2-AG, N-arachidonoyl glycine), and 4 related lipids (4 N-acylethanolamides) were measured in olfactory bulb, hypothalamus, hippocampus, thalamus, striatum, midbrain, cerebellum, and brainstem. Results. Overall, levels of these lipids were significantly lower among paced compared to standard mated rats with the most dramatic decreases observed in brainstem, hippocampus, midbrain, and striatum. However, chamber exposed rats had significantly higher levels of these lipids compared to home cage controls and paced mated wherein the hippocampus showed the largest increases. Conclusions. These data demonstrate that mating strategies and exposure to mating arenas influence lipid signaling in the brain.
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影响因子：
1.3
作者：
MCCLINTOCK, MK;ADLER, NT
通讯作者：
ADLER, NT
影响因子：
2.4
作者：
McHugh D;Hu SS;Rimmerman N;Juknat A;Vogel Z;Walker JM;Bradshaw HB
通讯作者：
Bradshaw HB
DOI：
10.1530/jrf.0.0900375
发表时间：
1990-11-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
FRYE, CA;ERSKINE, MS
通讯作者：
ERSKINE, MS
影响因子：
3.8
作者：
Frye, Cheryl A.;Paris, Jason J.;Rhodes, Madeline E.
通讯作者：
Rhodes, Madeline E.
影响因子：
3.5
作者：
Martínez, I;Paredes, RG
通讯作者：
Paredes, RG