Identification of long non-coding RNAs in the immature and mature rat anterior pituitary.
Identification of long non-coding RNAs in the immature and mature rat anterior pituitary.
复制标题
未成熟和成熟大鼠垂体前叶中长非编码RNA的鉴定
DOI：
10.1038/s41598-017-17996-6
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发表时间：
2017-12-19
影响因子：
4.6
通讯作者：
Zhang JB
中科院分区：
文献类型：
--
作者：
Han DX;Sun XL;Fu Y;Wang CJ;Liu JB;Jiang H;Gao Y;Chen CZ;Yuan B;Zhang JB
长链非编码RNA（longnoncodingRNA，lncRNA）在多种类型的垂体腺瘤和正常垂体前叶中被发现，其中一些与垂体腺瘤的发病机制有关。然而，在正常垂体的不同发育阶段，特别是在大鼠中，表达的lncRNA的系统分析尚未进行。因此，我们对比了在Illumina HiSeq Xten平台上测序的大鼠未成熟（D15）和成熟（D120）垂体前叶的两个cDNA文库，并且总共鉴定了29，568，806，352个干净读段。值得注意的是，7039个lncRNA转录本对应于4442个lncRNA基因，并且1181个lncRNA转录本在D15和D120中显著差异表达。此外，6839个蛋白质编码基因（上游和下游<100 kb）是4074个lncRNA基因的最近邻居。利用lncRNATargets平台构建了lncRNA与卵泡刺激素β亚基（FSHb）基因的相互作用网络，获得了3个新的lncRNA。此外，我们检测了新的lncRNA的表达和通过定量PCR（qPCR）随机选择的10个高表达lncRNA。本研究中鉴定的大鼠垂体前叶lncRNA含量提供了对这些lncRNA在哺乳动物激素和生殖发育和调节中的作用的更深入的理解。
Many long non-coding RNAs (lncRNAs) have been identified in several types of human pituitary adenomas and normal anterior pituitary, some of which are involved in the pathogenesis of pituitary adenomas. However, a systematic analysis of lncRNAs expressed at different developmental stages of normal pituitary, particularly in rats, has not been performed. Therefore, we contrasted two cDNA libraries of immature (D15) and mature (D120) anterior pituitary in rat that were sequenced on an Illumina HiSeq Xten platform, and a total of 29,568,806,352 clean reads were identified. Notably, 7039 lncRNA transcripts corresponded to 4442 lncRNA genes, and 1181 lncRNA transcripts were significantly differentially expressed in D15 and D120. In addition, 6839 protein-coding genes (<100 kb upstream and downstream) were the nearest neighbors of 4074 lncRNA genes. An interaction network of lncRNAs and the follicle-stimulating hormone beta-subunit (FSHb) gene was constructed using the lncRNATargets platform, and three novel lncRNAs were obtained. Furthermore, we detected the expression of the novel lncRNAs and ten highly expressed lncRNAs that were randomly selected through quantitative PCR (qPCR). The rat anterior pituitary lncRNA content identified in this study provides a more in-depth understanding of the roles of these lncRNAs in hormone and reproduction development and regulation in mammals.
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影响因子：
11.1
作者：
Bonasio R;Shiekhattar R
通讯作者：
Shiekhattar R
microRNA-21-3p和microRNA-433差异表达在大鼠垂体前叶细胞FSH调节中的作用
影响因子：
--
作者：
Han DX;Sun XL;Xu MQ;Chen CZ;Jiang H;Gao Y;Yuan B;Zhang JB
通讯作者：
Zhang JB
影响因子：
2.2
作者：
Binse, I.;Ueberberg, B.;Petersenn, S.
通讯作者：
Petersenn, S.
影响因子：
6.4
作者：
Cao J
通讯作者：
Cao J
影响因子：
8.8
作者：
Jiang W;Liu Y;Liu R;Zhang K;Zhang Y
通讯作者：
Zhang Y