The prevalence of the honeybee brood pathogens Ascosphaera apis, Paenibacillus larvae and Melissococcus plutonius in Spanish apiaries determined with a new multiplex PCR assay.
The prevalence of the honeybee brood pathogens Ascosphaera apis, Paenibacillus larvae and Melissococcus plutonius in Spanish apiaries determined with a new multiplex PCR assay.
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DOI：
10.1111/1751-7915.12070
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发表时间：
2013-11
影响因子：
5.7
通讯作者：
Martín-Hernández R
中科院分区：
文献类型：
--
作者：
Garrido-Bailón E;Higes M;Martínez-Salvador A;Antúnez K;Botías C;Meana A;Prieto L;Martín-Hernández R
蜜蜂囊腔菌、幼虫类芽孢杆菌和蜜蜂蜜球菌是影响蜜蜂育雏的三种最重要的病原菌。本研究的目的是评估这些病原体在蜂群中的流行情况，并阐明它们在西班牙蜂群损失中的作用。为了获得该基因，我们建立了一种多重PCR方法，同时扩增了幼虫和粘虫的16S核糖体核糖核酸基因。plutonius的5.8SrRNA基因，以及A. apis。多重PCR检测方法提供了一种快速、特异的方法，成功地检测了三种感染性病原体（幼虫P. plutonius和A. API），而不需要微生物培养。然后，这种技术被用来评估这些病原体在西班牙蜜蜂群在2006年和2007年的患病率，揭示了我们的研究结果，这些病原体在大多数地理区域的低患病率。
The microorganisms Ascosphaera apis, Paenibacillus larvae and Melissococcus plutonius are the three most important pathogens that affect honeybee brood. The aim of the present study was to evaluate the prevalence of these pathogens in honeybee colonies and to elucidate their role in the honeybee colony losses in Spain. In order to get it, a multiplex polymerase chain reaction (PCR) assay was developed to simultaneously amplify the16S ribosomal ribonucleic acid (rRNA) gene of P. larvae and M. plutonius, and the 5.8S rRNA gene of A. apis. The multiplex PCR assay provides a quick and specific tool that successfully detected the three infectious pathogens (P. larvae, M. plutonius and A. apis) in brood and adult honeybee samples without the need for microbiological culture. This technique was then used to evaluate the prevalence of these pathogens in Spanish honeybee colonies in 2006 and 2007, revealing our results a low prevalence of these pathogens in most of the geographic areas studied.
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影响因子：
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作者：
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通讯作者：
Ulgen, Mihriban
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